PDBsum entry 3v31

Protein binding

PDB id: 3v31

Contents

Protein chains

166 a.a.

18 a.a.

Metals

_NA ×2

_CL

Waters ×139

PDB id:

3v31

Name:

Protein binding

Title:

Crystal structure of the peptide bound complex of the ankyrin repeat domains of human ankra2

Structure:

Ankyrin repeat family a protein 2. Chain: a. Fragment: unp residues 148-313 (ank repeats). Synonym: rfxank-like protein 2. Engineered: yes. Histone deacetylase 4. Chain: b. Synonym: hd4. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ankra, ankra2. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Other_details: this sequence occurs naturally in human hdac4.

Resolution:

1.57Å R-factor: 0.176 R-free: 0.205

Authors:

R.Lam,C.Xu,C.B.Bian,J.Kania,C.Bountra,J.Weigelt,C.H.Arrowsmith, A.M.Edwards,A.Bochkarev,J.Min,Structural Genomics Consortium (Sgc)

Key ref:

C.Xu et al. (2012). Sequence-specific recognition of a PxLPxI/L motif by an ankyrin repeat tumbler lock. Sci Signal, 5, ra39. PubMed id: 22649097

Date:

12-Dec-11 Release date: 04-Apr-12

Protein chain

Q9H9E1  (ANRA2_HUMAN) -  Ankyrin repeat family A protein 2 from Homo sapiens

Seq: 313 a.a.

Struc: 166 a.a.*

Protein chain

P56524  (HDAC4_HUMAN) -  Histone deacetylase 4 from Homo sapiens

Seq: 1084 a.a.

Struc: 17 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain B: E.C.3.5.1.98 - histone deacetylase.
• Reaction: N₆-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

Sci Signal 5:ra39 (2012)

PubMed id: 22649097

Sequence-specific recognition of a PxLPxI/L motif by an ankyrin repeat tumbler lock.

C.Xu, J.Jin, C.Bian, R.Lam, R.Tian, R.Weist, L.You, J.Nie, A.Bochkarev, W.Tempel, C.S.Tan, G.A.Wasney, M.Vedadi, G.D.Gish, C.H.Arrowsmith, T.Pawson, X.J.Yang, J.Min.

ABSTRACT

No abstract given.