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PDBsum entry 3uzs
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protein dna_rna metals Protein-protein interface(s) links
Transferase/RNA PDB id
3uzs

 

 

 

 

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Contents
Protein chains
609 a.a.
339 a.a.
67 a.a.
DNA/RNA
Metals
_MG
PDB id:
3uzs
Name: Transferase/RNA
Title: Structure of the c13.28 RNA aptamer bound to the g protein-coupled receptor kinase 2-heterotrimeric g protein beta 1 and gamma 2 subunit complex
Structure: Beta-adrenergic receptor kinase 1. Chain: a. Synonym: beta-ark-1, g-protein-coupled receptor kinase 2. Engineered: yes. Mutation: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1. Chain: b. Synonym: transducin beta chain 1.
Source: Bos taurus. Bovine. Organism_taxid: 9913. Gene: adrbk1, grk2. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: gnb1. Expressed in: trichoplusia ni. Expression_system_taxid: 7111.
Resolution:
4.52Å     R-factor:   0.188     R-free:   0.268
Authors: J.J.G.Tesmer,V.M.Tesmer
Key ref: V.M.Tesmer et al. (2012). Molecular mechanism for inhibition of g protein-coupled receptor kinase 2 by a selective RNA aptamer. Structure, 20, 1300-1309. PubMed id: 22727813
Date:
07-Dec-11     Release date:   11-Jul-12    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P21146  (ARBK1_BOVIN) -  Beta-adrenergic receptor kinase 1 from Bos taurus
Seq:
Struc: 		 
Seq:
Struc: 		689 a.a.
609 a.a.*
Protein chain
Pfam   ArchSchema ?
P62871  (GBB1_BOVIN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Bos taurus
Seq:
Struc: 		340 a.a.
339 a.a.
Protein chain
Pfam   ArchSchema ?
P63212  (GBG2_BOVIN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Bos taurus
Seq:
Struc: 		71 a.a.
67 a.a.*
Key:    PfamA domain  Secondary structure
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DNA/RNA chain
  G-G-C-A-G-A-C-C-A-U-A-C-G-G-G-U-U-G-C-C 20 bases

 Enzyme reactions 
   Enzyme class: Chain A: E.C.2.7.11.15  - [beta-adrenergic-receptor] kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: [beta-adrenergic receptor] + ATP = [beta-adrenergic receptor]-phosphate + ADP + H+
[beta-adrenergic receptor]
 + ATP
 = [beta-adrenergic receptor]-phosphate
 + ADP
 + H(+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
Structure 20:1300-1309 (2012)
PubMed id: 22727813  
 
 
Molecular mechanism for inhibition of g protein-coupled receptor kinase 2 by a selective RNA aptamer.
V.M.Tesmer, S.Lennarz, G.Mayer, J.J.Tesmer.
 
  ABSTRACT  
 
No abstract given.

 

 

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