PDBsum entry 3upu

Hydrolase/DNA

PDB id: 3upu

Contents

Protein chains

439 a.a.

DNA/RNA

Waters ×10

PDB id:

3upu

Name:

Hydrolase/DNA

Title:

Crystal structure of the t4 phage sf1b helicase dda

Structure:

Atp-dependent DNA helicase dda. Chain: a, b, c. Synonym: sf1b helicase dda. Engineered: yes. Mutation: yes. 5'-d( Tp Tp Tp Tp Tp Tp Tp T)-3'. Chain: d, e, f. Engineered: yes

Source:

Enterobacteria phage t4. Organism_taxid: 10665. Gene: dda, sud. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes

Resolution:

3.30Å R-factor: 0.207 R-free: 0.258

Authors:

X.He,M.K.Yun,C.W.Pemble Iv,K.N.Kreuzer,K.D.Raney,S.W.White

Key ref:

X.He et al. (2012). The T4 phage SF1B helicase Dda is structurally optimized to perform DNA strand separation. Structure, 20, 1189-1200. PubMed id: 22658750

Date:

18-Nov-11 Release date: 20-Jun-12

Protein chains

P32270  (DDA_BPT4) -  ATP-dependent DNA helicase dda from Enterobacteria phage T4

Seq: 439 a.a.

Struc: 439 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

DNA/RNA chains

T-T-T-T-T-T-T-T 8 bases

T-T-T-T-T-T-T-T 8 bases

T-T-T-T-T-T-T-T 8 bases

Enzyme reactions

• Enzyme class: E.C.3.6.4.12 - Dna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Structure 20:1189-1200 (2012)

PubMed id: 22658750

The T4 phage SF1B helicase Dda is structurally optimized to perform DNA strand separation.

X.He, A.K.Byrd, M.K.Yun, C.W.Pemble, D.Harrison, L.Yeruva, C.Dahl, K.N.Kreuzer, K.D.Raney, S.W.White.

ABSTRACT

No abstract given.