PDBsum entry 3ulr

Hydrolase, protein binding

PDB id: 3ulr

Contents

Protein chains

129 a.a.

64 a.a.

13 a.a.

Waters ×292

PDB id:

3ulr

Name:

Hydrolase, protein binding

Title:

Lysozyme contamination facilitates crystallization of a hetero- trimericcortactin:arg:lysozyme complex

Structure:

LysozymE C. Chain: a. Synonym: 1,4-beta-n-acetylmuramidasE C, allergen gal d iv. Engineered: yes. Src substrate cortactin. Chain: b. Fragment: sh3 domain (unp residues 487-546). Engineered: yes. Abelson tyrosine-protein kinase 2.

Source:

Gallus gallus. Bantam,chickens. Organism_taxid: 9031. Gene: lyz. Mus musculus. Mouse. Organism_taxid: 10090. Gene: cttn, ems1. Expressed in: escherichia coli.

Resolution:

1.65Å R-factor: 0.186 R-free: 0.225

Authors:

W.Liu,S.Macgrath,A.J.Koleske,T.J.Boggon

Key ref:

W.Liu et al. (2012). Lysozyme contamination facilitates crystallization of a heterotrimeric cortactin-Arg-lysozyme complex. Acta Crystallogr Sect F Struct Biol Cryst Commun, 68, 154-158. PubMed id: 22297987 DOI: 10.1107/S1744309111056132

Date:

11-Nov-11 Release date: 11-Jan-12

Protein chain

P00698  (LYSC_CHICK) -  Lysozyme C from Gallus gallus

Seq: 147 a.a.

Struc: 129 a.a.

Protein chain

Q60598  (SRC8_MOUSE) -  Src substrate cortactin from Mus musculus

Seq: 546 a.a.

Struc: 64 a.a.*

Protein chain

P42684  (ABL2_HUMAN) -  Tyrosine-protein kinase ABL2 from Homo sapiens

Seq: 1182 a.a.

Struc: 13 a.a.

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chain A: E.C.3.2.1.17 - lysozyme.
• Reaction: Hydrolysis of the 1,4-beta-linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan heteropolymers of the prokaryotes cell walls.
• Enzyme class 3: Chain C: E.C.2.7.10.2 - non-specific protein-tyrosine kinase.
• Reaction: L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1107/S1744309111056132

Acta Crystallogr Sect F Struct Biol Cryst Commun 68:154-158 (2012)

PubMed id: 22297987

Lysozyme contamination facilitates crystallization of a heterotrimeric cortactin-Arg-lysozyme complex.

W.Liu, S.M.MacGrath, A.J.Koleske, T.J.Boggon.

ABSTRACT

No abstract given.