PDBsum entry 3ul7

Immune system

PDB id: 3ul7

Contents

Protein chain

276 a.a.

Ligands

NAG-NAG-BMA

NAG

FUL

SO4 ×3

Waters ×50

PDB id:

3ul7

Name:

Immune system

Title:

Crystal structure of the tv3 mutant f63w

Structure:

Toll-like receptor 4, variable lymphocyte receptor b. Chain: a. Fragment: unp residues 28-228, unp residues 126-199. Synonym: htoll. Engineered: yes. Mutation: yes. Other_details: the fusion protein of tlr4 (unp residues 28-228) and vlrb (unp residues 126-199)

Source:

Homo sapiens, eptatretus burgeri. Human, inshore hagfish. Organism_taxid: 9606, 7764. Gene: tlr4, vlrb. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108.

Resolution:

2.37Å R-factor: 0.231 R-free: 0.271

Authors:

H.J.Kim,H.K.Cheong,Y.H.Jeon

Key ref:

J.Han et al. (2012). Structure-based rational design of a Toll-like receptor 4 (TLR4) decoy receptor with high binding affinity for a target protein. Plos One, 7, e30929. PubMed id: 22363519

Date:

10-Nov-11 Release date: 04-Apr-12

Protein chain

O00206  (TLR4_HUMAN) -  Toll-like receptor 4 from Homo sapiens

Seq: 839 a.a.

Struc: 276 a.a.*

Protein chain

Q4G1L2  (Q4G1L2_EPTBU) -  Variable lymphocyte receptor B from Eptatretus burgeri

Seq: 273 a.a.

Struc: 276 a.a.*

* PDB and UniProt seqs differ at 169 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.2.2.6 - ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase.
• Reaction: NAD⁺ + H2O = ADP-D-ribose + nicotinamide + H⁺

NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+) (Bound ligand (Het Group name = NAG) matches with 43.75% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Plos One 7:e30929 (2012)

PubMed id: 22363519

Structure-based rational design of a Toll-like receptor 4 (TLR4) decoy receptor with high binding affinity for a target protein.

J.Han, H.J.Kim, S.C.Lee, S.Hong, K.Park, Y.H.Jeon, D.Kim, H.K.Cheong, H.S.Kim.

ABSTRACT

No abstract given.