PDBsum entry 3ujg

Signaling protein

PDB id: 3ujg

Contents

Protein chains

290 a.a.

315 a.a.

Ligands

SO4 ×3

Metals

_MG ×2

Waters ×146

PDB id:

3ujg

Name:

Signaling protein

Title:

Crystal structure of snrk2.6 in complex with hab1

Structure:

Serine/threonine-protein kinase srk2e. Chain: a. Fragment: kinase domain. Synonym: protein open stomata 1, snf1-related kinase 2.6, snrk2.6, serine/threonine-protein kinase ost1. Engineered: yes. Mutation: yes. Protein phosphatase 2c 16. Chain: b.

Source:

Arabidopsis thaliana. Mouse-ear cress,thale-cress. Organism_taxid: 3702. Gene: srk2e, ost1, snrk2.6, at4g33950, f17i5.140. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: hab1, p2c-ha, at1g72770, f28p22.4.

Resolution:

2.60Å R-factor: 0.209 R-free: 0.238

Authors:

X.E.Zhou,F.-F.Soon,L.-M.Ng,A.Kovach,M.H.E.Tan,K.M.Suino-Powell,Y.He, Y.Xu,J.S.Brunzelle,J.Li,K.Melcher,H.E.Xu

Key ref:

F.F.Soon et al. (2012). Molecular mimicry regulates ABA signaling by SnRK2 kinases and PP2C phosphatases. Science, 335, 85-88. PubMed id: 22116026

Date:

07-Nov-11 Release date: 15-Feb-12

Protein chain

Q940H6  (SRK2E_ARATH) -  Serine/threonine-protein kinase SRK2E from Arabidopsis thaliana

Seq: 362 a.a.

Struc: 290 a.a.*

Protein chain

Q9CAJ0  (P2C16_ARATH) -  Protein phosphatase 2C 16 from Arabidopsis thaliana

Seq: 511 a.a.

Struc: 315 a.a.

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: Chain A: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺
• Enzyme class 2: Chain B: E.C.3.1.3.16 - protein-serine/threonine phosphatase.
• Reaction:
  1. O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate
  2. O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Science 335:85-88 (2012)

PubMed id: 22116026

Molecular mimicry regulates ABA signaling by SnRK2 kinases and PP2C phosphatases.

F.F.Soon, L.M.Ng, X.E.Zhou, G.M.West, A.Kovach, M.H.Tan, K.M.Suino-Powell, Y.He, Y.Xu, M.J.Chalmers, J.S.Brunzelle, H.Zhang, H.Yang, H.Jiang, J.Li, E.L.Yong, S.Cutler, J.K.Zhu, P.R.Griffin, K.Melcher, H.E.Xu.

ABSTRACT

No abstract given.