PDBsum entry 3uc3

Transferase

PDB id: 3uc3

Contents

Protein chain

272 a.a.

Metals

_CO ×3

Waters ×143

PDB id:

3uc3

Name:

Transferase

Title:

The crystal structure of snf1-related kinase 2.3

Structure:

Serine/threonine-protein kinase srk2i. Chain: a. Fragment: kinase domain. Synonym: ost1-kinase-like 2, protein athprokin b, snf1-related kinase 2.3, snrk2.3. Engineered: yes. Mutation: yes

Source:

Arabidopsis thaliana. Mouse-ear cress,thale-cress. Organism_taxid: 3702. Gene: srk2i, 41k, oskl2, snrk2.3, at5g66880, mud21.14. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.90Å R-factor: 0.214 R-free: 0.230

Authors:

X.E.Zhou,L.-M.Ng,F.-F.Soon,A.Kovach,K.M.Suino-Powell,J.Li,K.Melcher, H.E.Xu

Key ref:

L.M.Ng et al. (2011). Structural basis for basal activity and autoactivation of abscisic acid (ABA) signaling SnRK2 kinases. Proc Natl Acad Sci U S A, 108, 21259-21264. PubMed id: 22160701

Date:

25-Oct-11 Release date: 14-Dec-11

Protein chain

Q39193  (SRK2I_ARATH) -  Serine/threonine-protein kinase SRK2I from Arabidopsis thaliana

Seq: 361 a.a.

Struc: 272 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Proc Natl Acad Sci U S A 108:21259-21264 (2011)

PubMed id: 22160701

Structural basis for basal activity and autoactivation of abscisic acid (ABA) signaling SnRK2 kinases.

L.M.Ng, F.F.Soon, X.E.Zhou, G.M.West, A.Kovach, K.M.Suino-Powell, M.J.Chalmers, J.Li, E.L.Yong, J.K.Zhu, P.R.Griffin, K.Melcher, H.E.Xu.

ABSTRACT

No abstract given.