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PDBsum entry 3tvj
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protein ligands Protein-protein interface(s) links
Hydrolase PDB id
3tvj

 

 

 

 

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Contents
Protein chains
83 a.a.
242 a.a.
35 a.a.
Ligands
SO4
Waters ×534
PDB id:
3tvj
Name: Hydrolase
Title: Catalytic fragment of masp-2 in complex with its specific inhibitor developed by directed evolution on sgci scaffold
Structure: Mannan-binding lectin serine protease 2 a chain. Chain: a. Fragment: sushi 2 domain residues 363-444. Synonym: mannan-binding lectin serine protease 2, mbl-associated serine protease 2, mannose-binding protein-associated serine protease 2, masp-2. Engineered: yes. Mannan-binding lectin serine protease 2 b chain. Chain: b.
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: masp2. Expressed in: escherichia coli. Expression_system_taxid: 469008. Schistocerca gregaria. Desert locust. Organism_taxid: 7010.
Resolution:
1.28Å     R-factor:   0.159     R-free:   0.205
Authors: D.Heja,V.Harmat,J.Dobo,R.Szasz,K.A.Kekesi,P.Zavodszky,P.Gal,G.Pal
Key ref: D.Héja et al. (2012). Monospecific inhibitors show that both mannan-binding lectin-associated serine protease-1 (MASP-1) and -2 Are essential for lectin pathway activation and reveal structural plasticity of MASP-2. J Biol Chem, 287, 20290-20300. PubMed id: 22511776
Date:
20-Sep-11     Release date:   25-Apr-12    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
O00187  (MASP2_HUMAN) -  Mannan-binding lectin serine protease 2 from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		686 a.a.
83 a.a.*
Protein chain
Pfam   ArchSchema ?
O00187  (MASP2_HUMAN) -  Mannan-binding lectin serine protease 2 from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		686 a.a.
242 a.a.
Protein chain
Pfam   ArchSchema ?
O46162  (SGP1_SCHGR) -  Serine protease inhibitor I/II from Schistocerca gregaria
Seq:
Struc: 		92 a.a.
35 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 7 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chains A, B: E.C.3.4.21.104  - mannan-binding lectin-associated serine protease-2.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

 

 
J Biol Chem 287:20290-20300 (2012)
PubMed id: 22511776  
 
 
Monospecific inhibitors show that both mannan-binding lectin-associated serine protease-1 (MASP-1) and -2 Are essential for lectin pathway activation and reveal structural plasticity of MASP-2.
D.Héja, V.Harmat, K.Fodor, M.Wilmanns, J.Dobó, K.A.Kékesi, P.Závodszky, P.Gál, G.Pál.
 
  ABSTRACT  
 
No abstract given.

 

 

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