PDBsum entry 3tuz

Hydrolase/transport protein

PDB id: 3tuz

Contents

Protein chains

216 a.a.

344 a.a.

Ligands

MSE ×4

ADP ×4

PDB id:

3tuz

Name:

Hydrolase/transport protein

Title:

Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form

Structure:

D-methionine transport system permease protein meti. Chain: a, b, e, f. Engineered: yes. Methionine import atp-binding protein metn. Chain: c, d, g, h. Engineered: yes. Mutation: yes

Source:

Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: b0198, jw0194, meti, yaee. Expressed in: escherichia coli. Expression_system_taxid: 511693. Gene: abc, b0199, jw0195, metn.

Resolution:

3.40Å R-factor: 0.263 R-free: 0.306

Authors:

E.Johnson,P.Nguyen,D.C.Rees

Key ref:

E.Johnson et al. (2012). Inward facing conformations of the MetNI methionine ABC transporter: Implications for the mechanism of transinhibition. Protein Sci, 21, 84-96. PubMed id: 22095702

Date:

19-Sep-11 Release date: 30-Nov-11

Protein chains

P31547  (METI_ECOLI) -  D-methionine transport system permease protein MetI from Escherichia coli (strain K12)

Seq: 217 a.a.

Struc: 216 a.a.

Protein chains

P30750  (METN_ECOLI) -  Methionine import ATP-binding protein MetN from Escherichia coli (strain K12)

Seq: 343 a.a.

Struc: 344 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains C, D, G, H: E.C.7.4.2.11 - ABC-type methionine transporter.
• Reaction:
  1. L-methionine(out) + ATP + H2O = L-methionine(in) + ADP + phosphate + H⁺
  2. D-methionine(out) + ATP + H2O = D-methionine(in) + ADP + phosphate + H⁺

L-methionine(out) + ATP + H2O = L-methionine(in) (Bound ligand (Het Group name = ADP) corresponds exactly) + ADP + phosphate + H(+)

D-methionine(out) + ATP + H2O = D-methionine(in) (Bound ligand (Het Group name = ADP) corresponds exactly) + ADP + phosphate + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Protein Sci 21:84-96 (2012)

PubMed id: 22095702

Inward facing conformations of the MetNI methionine ABC transporter: Implications for the mechanism of transinhibition.

E.Johnson, P.T.Nguyen, T.O.Yeates, D.C.Rees.

ABSTRACT

No abstract given.