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PDBsum entry 3tu4
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protein dna_rna Protein-protein interface(s) links
Signaling protein/structural protein/DNA PDB id
3tu4

 

 

 

 

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Contents
Protein chains
98 a.a.
89 a.a.
107 a.a.
93 a.a.
214 a.a.
DNA/RNA
PDB id:
3tu4
Name: Signaling protein/structural protein/DNA
Title: Crystal structure of the sir3 bah domain in complex with a nucleosome core particle.
Structure: Histone h3.2. Chain: a, e. Engineered: yes. Histone h4. Chain: b, f. Engineered: yes. Histone h2a. Chain: c, g. Engineered: yes.
Source: Xenopus laevis. Clawed frog,common platanna,platanna. Organism_taxid: 8355. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: hist1h2aj, loc494591. Synthetic: yes. Other_details: synthetic construct. Saccharomyces cerevisiae s288c.
Resolution:
3.00Å     R-factor:   0.193     R-free:   0.241
Authors: K.-J.Armache,J.D.Garlick,D.Canzio,G.J.Narlikar,R.E.Kingston
Key ref: K.J.Armache et al. (2011). Structural basis of silencing: Sir3 BAH domain in complex with a nucleosome at 3.0 Å resolution. Science, 334, 977-982. PubMed id: 22096199
Date:
15-Sep-11     Release date:   23-Nov-11    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P84233  (H32_XENLA) -  Histone H3.2 from Xenopus laevis
Seq:
Struc: 		136 a.a.
98 a.a.*
Protein chains
Pfam   ArchSchema ?
P62799  (H4_XENLA) -  Histone H4 from Xenopus laevis
Seq:
Struc: 		103 a.a.
89 a.a.
Protein chains
Pfam   ArchSchema ?
P06897  (H2A1_XENLA) -  Histone H2A type 1 from Xenopus laevis
Seq:
Struc: 		130 a.a.
107 a.a.*
Protein chains
Pfam   ArchSchema ?
P02281  (H2B11_XENLA) -  Histone H2B 1.1 from Xenopus laevis
Seq:
Struc: 		126 a.a.
93 a.a.*
Protein chains
Pfam   ArchSchema ?
P06701  (SIR3_YEAST) -  Regulatory protein SIR3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Seq:
Struc: 		 
Seq:
Struc: 		978 a.a.
214 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 5 residue positions (black crosses)

DNA/RNA chains
  T-C-G-A-G-A-A-T-C-C-C-G-G-T-G-C-C-G-A-G-G-C-C-G-C-T-C-A-A-T-T-G-G-T-C-G-T-A-G- 146 bases
  A-T-C-G-G-A-T-G-T-A-T-A-T-A-T-C-T-G-A-C-A-C-G-T-G-C-C-T-G-G-A-G-A-C-T-A-G-G-G- 146 bases

 Enzyme reactions 
   Enzyme class: Chains A, B, C, D, E, F, G, H, K, L: E.C.?
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

 

 
Science 334:977-982 (2011)
PubMed id: 22096199  
 
 
Structural basis of silencing: Sir3 BAH domain in complex with a nucleosome at 3.0 Å resolution.
K.J.Armache, J.D.Garlick, D.Canzio, G.J.Narlikar, R.E.Kingston.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
22398447 A.J.Kuo, J.Song, P.Cheung, S.Ishibe-Murakami, S.Yamazoe, J.K.Chen, D.J.Patel, and O.Gozani (2012).
The BAH domain of ORC1 links H4K20me2 to DNA replication licensing and Meier-Gorlin syndrome.
  Nature, 484, 115-119.
PDB codes: 4dov 4dow
22722606 K.Luger, M.L.Dechassa, and D.J.Tremethick (2012).
New insights into nucleosome and chromatin structure: an ordered state or a disordered affair?
  Nat Rev Mol Cell Biol, 13, 436-447.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.

 

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