PDBsum entry 3tnq

Transferase

PDB id

3tnq

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Contents

			Protein chains

					334 a.a.


					270 a.a.

			Ligands

					ADP

			Metals

					_MG ×2

			Waters ×13

PDB id:

3tnq

Links

Name:

Transferase

Title:

Structure and allostery of the pka riib tetrameric holoenzyme

Structure:

Protein kinase, camp-dependent, catalytic, alpha. Chain: b. Engineered: yes. Camp-dependent protein kinase type ii-beta regulatory subunit. Chain: a. Engineered: yes. Mutation: yes

Source:

Rattus norvegicus. Rat. Organism_taxid: 10116. Gene: prkaca, rcg_51506. Expressed in: escherichia coli. Expression_system_taxid: 562. Mus musculus. Mouse. Organism_taxid: 10090.

Resolution:

3.10Å

R-factor:

0.234

R-free:

0.275

Authors:

P.Zhang,E.V.Smith-Nguyen,M.M.Keshwani,M.S.Deal,A.P.Kornev,S.S.Taylor

Key ref:

P.Zhang et al. (2012). Structure and allostery of the PKA RIIβ tetrameric holoenzyme. Science, 335, 712-716. PubMed id: 22323819

Date:

01-Sep-11

Release date:

01-Feb-12

PROCHECK

	Headers

	References

Protein chain

P27791 (KAPCA_RAT) - cAMP-dependent protein kinase catalytic subunit alpha from Rattus norvegicus

Seq: Struc:					351 a.a. 334 a.a.*

Protein chain

P31324 (KAP3_MOUSE) - cAMP-dependent protein kinase type II-beta regulatory subunit from Mus musculus

Seq: Struc:					416 a.a. 270 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 5 residue positions (black crosses)



		Enzyme reactions

Enzyme class:

Chain B: E.C.2.7.11.11 - cAMP-dependent protein kinase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

1.	L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
2.	L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

L-seryl-[protein]	+	ATP	=	O-phospho-L-seryl-[protein] Bound ligand (Het Group name = ADP) corresponds exactly	+	ADP	+	H(+)

L-threonyl-[protein]	+	ATP	=	O-phospho-L-threonyl-[protein] Bound ligand (Het Group name = ADP) corresponds exactly	+	ADP	+	H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

	Science 335:712-716 (2012)
PubMed id: 22323819

Structure and allostery of the PKA RIIβ tetrameric holoenzyme.
P.Zhang, E.V.Smith-Nguyen, M.M.Keshwani, M.S.Deal, A.P.Kornev, S.S.Taylor.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

22992589

S.S.Taylor, R.Ilouz, P.Zhang, and A.P.Kornev (2012).
Assembly of allosteric macromolecular switches: lessons from PKA.

Nat Rev Mol Cell Biol, 13, 646-658.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.