UniProt functional annotation for Q96G74



	UniProt functional annotation for Q96G74

UniProt code: Q96G74.

Organism: Homo sapiens (Human).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.

Function: Deubiquitinating enzyme that functions as negative regulator of the innate immune system. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production. Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro). {ECO:0000269|PubMed:17991829, ECO:0000269|PubMed:22245969, ECO:0000269|PubMed:23827681}.

Catalytic activity: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76- residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000269|PubMed:22245969};

Activity regulation: Inhibited by N-ethyl-maleimide (NEM).

Subunit: Interacts with TRAF3. {ECO:0000269|PubMed:17991829, ECO:0000269|PubMed:22245969}.

Tissue specificity: Expressed in various tissues, including the liver and placenta, as well as in peripheral blood leukocytes. {ECO:0000269|PubMed:17991829}.

Induction: Up-regulated by bacterial lipopolysaccharide (LPS) in bone marrow-derived macrophages. {ECO:0000269|PubMed:22245969}.

Ptm: Phosphorylation at Ser-177 is required for deubiquitinating activity. {ECO:0000269|PubMed:22245969}.

Miscellaneous: [Isoform 2]: Dubious isoform produced through aberrant splice sites. {ECO:0000305}.

Miscellaneous: [Isoform 3]: Dubious isoform produced through aberrant splice sites. {ECO:0000305}.

Similarity: Belongs to the peptidase C85 family. {ECO:0000305}.

Sequence caution: Sequence=BAB14131.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305}; Sequence=BAB15416.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Homo sapiens (Human).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.



Function:		Deubiquitinating enzyme that functions as negative regulator of the innate immune system. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production. Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro). {ECO:0000269\|PubMed:17991829, ECO:0000269\|PubMed:22245969, ECO:0000269\|PubMed:23827681}.


Catalytic activity:		Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76- residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000269\|PubMed:22245969};
Activity regulation:		Inhibited by N-ethyl-maleimide (NEM).
Subunit:		Interacts with TRAF3. {ECO:0000269\|PubMed:17991829, ECO:0000269\|PubMed:22245969}.
Tissue specificity:		Expressed in various tissues, including the liver and placenta, as well as in peripheral blood leukocytes. {ECO:0000269\|PubMed:17991829}.
Induction:		Up-regulated by bacterial lipopolysaccharide (LPS) in bone marrow-derived macrophages. {ECO:0000269\|PubMed:22245969}.
Ptm:		Phosphorylation at Ser-177 is required for deubiquitinating activity. {ECO:0000269\|PubMed:22245969}.
Miscellaneous:		[Isoform 2]: Dubious isoform produced through aberrant splice sites. {ECO:0000305}.
Miscellaneous:		[Isoform 3]: Dubious isoform produced through aberrant splice sites. {ECO:0000305}.
Similarity:		Belongs to the peptidase C85 family. {ECO:0000305}.
Sequence caution:		Sequence=BAB14131.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305}; Sequence=BAB15416.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};