PDBsum entry 3tix

Gene regulation/protein binding

PDB id: 3tix

Contents

Protein chains

153 a.a.

421 a.a.

Metals

_CL ×8

__K ×3

Waters ×26

PDB id:

3tix

Name:

Gene regulation/protein binding

Title:

Crystal structure of the chp1-tas3 complex core

Structure:

Ubiquitin-like protein smt3,RNA-induced transcriptional silencing complex protein tas3. Chain: a, c. Fragment: n-terminal domain,n-terminal domain. Synonym: rits protein tas3. Engineered: yes. Chromo domain-containing protein 1. Chain: b, d. Fragment: c-terminal half.

Source:

Saccharomyces cerevisiae, schizosaccharomyces pombe. Baker's yeast, fission yeast. Organism_taxid: 559292, 284812. Strain: atcc 204508 / s288c, 972 / atcc 24843. Gene: smt3, ydr510w, d9719.15, tas3, spbc83.03c. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Schizosaccharomyces pombe (strain 972 / atcc

Resolution:

2.90Å R-factor: 0.212 R-free: 0.243

Authors:

T.Schalch,L.Joshua-Tor

Key ref:

T.Schalch et al. (2011). The Chp1-Tas3 core is a multifunctional platform critical for gene silencing by RITS. Nat Struct Biol, 18, 1351-1357. PubMed id: 22081013

Date:

22-Aug-11 Release date: 16-Nov-11

Protein chains

O94687  (TAS3_SCHPO) -  RNA-induced transcriptional silencing complex protein tas3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

Seq: 549 a.a.

Struc: 153 a.a.*

Protein chains

Q12306  (SMT3_YEAST) -  Ubiquitin-like protein SMT3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 101 a.a.

Struc: 153 a.a.*

Protein chains

Q10103  (CHP1_SCHPO) -  Chromo domain-containing protein 1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

Seq: 960 a.a.

Struc: 421 a.a.

* PDB and UniProt seqs differ at 13 residue positions (black crosses)

Nat Struct Biol 18:1351-1357 (2011)

PubMed id: 22081013

The Chp1-Tas3 core is a multifunctional platform critical for gene silencing by RITS.

T.Schalch, G.Job, S.Shanker, J.F.Partridge, L.Joshua-Tor.

ABSTRACT

No abstract given.