PDBsum entry 3t25

Hydrolase

PDB id: 3t25

Contents

Protein chain

223 a.a.

Ligands

SO4

BEN

TMO ×3

Metals

_CA

Waters ×160

PDB id:

3t25

Name:

Hydrolase

Title:

Tmao-grown orthorhombic trypsin (bovine)

Structure:

Cationic trypsin. Chain: a. Synonym: beta-trypsin. Ec: 3.4.21.4

Source:

Bos taurus. Bovine,cow,domestic cattle,domestic cow. Organism_taxid: 9913

Resolution:

1.70Å R-factor: 0.158 R-free: 0.188

Authors:

J.Cahn,M.Venkat,H.Marshall,D.Juers

Key ref:

H.Marshall et al. (2012). The use of trimethylamine N-oxide as a primary precipitating agent and related methylamine osmolytes as cryoprotective agents for macromolecular crystallography. Acta Crystallogr D Biol Crystallogr, 68, 69-81. PubMed id: 22194335

Date:

22-Jul-11 Release date: 28-Dec-11

Protein chain

P00760  (TRY1_BOVIN) -  Serine protease 1 from Bos taurus

Seq: 246 a.a.

Struc: 223 a.a.

Enzyme reactions

• Enzyme class: E.C.3.4.21.4 - trypsin.
• Reaction: Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Acta Crystallogr D Biol Crystallogr 68:69-81 (2012)

PubMed id: 22194335

The use of trimethylamine N-oxide as a primary precipitating agent and related methylamine osmolytes as cryoprotective agents for macromolecular crystallography.

H.Marshall, M.Venkat, N.S.Seng, J.Cahn, D.H.Juers.

ABSTRACT

No abstract given.