PDBsum entry 3sqx

Hydrolase/RNA

PDB id

3sqx

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Contents

			Protein chain

					508 a.a.

			DNA/RNA

			Ligands

					ANP

			Metals

					_MG

			Waters ×103

PDB id:

3sqx

Links

Name:

Hydrolase/RNA

Title:

Structure of mss116p (nte and c-tail double deletion) bound to ssrna and amp-pnp

Structure:

Atp-dependent RNA helicase mss116, mitochondrial. Chain: a. Fragment: unp residues 88-597. Engineered: yes. RNA (5'-r( Up Up Up Up Up Up Up Up Up U)-3'). Chain: b. Engineered: yes

Source:

Saccharomyces cerevisiae s288c. Baker's yeast. Organism_taxid: 559292. Strain: atcc 204508 / s288c. Gene: mss116, yd9346.05c, ydr194c. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Other_details: synthesized

Resolution:

2.11Å

R-factor:

0.217

R-free:

0.260

Authors:

M.Del Campo,A.M.Lambowitz

Key ref:

G.Mohr et al. (2011). High-throughput genetic identification of functionally important regions of the yeast DEAD-box protein Mss116p. J Mol Biol, 413, 952-972. PubMed id: 21945532

Date:

06-Jul-11

Release date:

12-Oct-11

PROCHECK

	Headers

	References

Protein chain

P15424 (MS116_YEAST) - ATP-dependent RNA helicase MSS116, mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: Struc:

Seq: Struc:					664 a.a. 508 a.a.

Key:

PfamA domain

Secondary structure

CATH domain

DNA/RNA chain

U-U-U-U-U-U-U-U 8 bases



		Enzyme reactions

Enzyme class:

E.C.3.6.4.13 - Rna helicase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

ATP + H2O = ADP + phosphate + H⁺

ATP

H2O

ADP
Bound ligand (Het Group name = ANP)
matches with 81.25% similarity

phosphate

H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

	J Mol Biol 413:952-972 (2011)
PubMed id: 21945532

High-throughput genetic identification of functionally important regions of the yeast DEAD-box protein Mss116p.
G.Mohr, M.Del Campo, K.G.Turner, B.Gilman, R.Z.Wolf, A.M.Lambowitz.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

22940866

A.L.Mallam, M.Del Campo, B.Gilman, D.J.Sidote, and A.M.Lambowitz (2012).
Structural basis for RNA-duplex recognition and unwinding by the DEAD-box helicase Mss116p.

Nature, 490, 121-125.

PDB codes:

4db2 4db4

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.