PDBsum entry 3shr

Transferase

PDB id: 3shr

Contents

Protein chains

278 a.a.

Ligands

CMP ×2

SO4 ×2

PDB id:

3shr

Name:

Transferase

Title:

Crystal structure of cgmp-dependent protein kinase reveals novel site of interchain communication

Structure:

Cgmp-dependent protein kinase 1. Chain: a, b. Fragment: unp residues 79-356. Synonym: cgk 1, cgk1, cgmp-dependent protein kinase i, cgki. Engineered: yes

Source:

Bos taurus. Bovine,cow,domestic cattle,domestic cow. Organism_taxid: 9913. Gene: prkg1, prkg1b, prkgr1a, prkgr1b. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.50Å R-factor: 0.221 R-free: 0.277

Authors:

B.W.Osborne,J.Wu,S.S.Taylor,W.R.Dostmann

Key ref:

B.W.Osborne et al. (2011). Crystal structure of cGMP-dependent protein kinase reveals novel site of interchain communication. Structure, 19, 1317-1327. PubMed id: 21893290

Date:

16-Jun-11 Release date: 21-Sep-11

Protein chains

P00516  (KGP1_BOVIN) -  cGMP-dependent protein kinase 1 from Bos taurus

Seq: 671 a.a.

Struc: 278 a.a.

Enzyme reactions

• Enzyme class: E.C.2.7.11.12 - cGMP-dependent protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] (Bound ligand (Het Group name = CMP) matches with 81.48% similarity) + ADP + H(+)

L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] (Bound ligand (Het Group name = CMP) matches with 81.48% similarity) + ADP + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Structure 19:1317-1327 (2011)

PubMed id: 21893290

Crystal structure of cGMP-dependent protein kinase reveals novel site of interchain communication.

B.W.Osborne, J.Wu, C.J.McFarland, C.K.Nickl, B.Sankaran, D.E.Casteel, V.L.Woods, A.P.Kornev, S.S.Taylor, W.R.Dostmann.

ABSTRACT

No abstract given.