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PDBsum entry 3sgb
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Complex(serine proteinase-inhibitor)
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PDB id
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3sgb
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Structure of the complex of streptomyces griseus protease b and the third domain of the turkey ovomucoid inhibitor at 1.8-A resolution.
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Authors
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R.J.Read,
M.Fujinaga,
A.R.Sielecki,
M.N.James.
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Ref.
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Biochemistry, 1983,
22,
4420-4433.
[DOI no: ]
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PubMed id
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Abstract
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The structure of the complex between the serine protease Streptomyces griseus
protease B (SGPB) and the third domain of the Kazal-type ovomucoid inhibitor
from turkey has been solved at 1.8-A resolution and refined to a conventional R
factor of 0.125. As others have reported previously for analogous complexes of
proteases and protein inhibitors, the inhibitor binds in a fashion similar to
that of a substrate; it is not cleaved, but there is a close approach (2.7 A) of
the active site nucleophile Ser-195 O gamma to the carbonyl carbon of the
reactive peptide bond of the inhibitor. Contrary to the structural reports
regarding the other enzyme-inhibitor complexes, we conclude that there is no
evidence for a significant distortion of this peptide bond from planarity. The
mechanism of inhibition can be understood in terms of the equilibrium
thermodynamic parameters Ka, the enzyme-inhibitor association constant, and
Khyd, the equilibrium constant for inhibitor hydrolysis. These thermodynamic
parameters can be rationalized in terms of the observed structure.
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Secondary reference #1
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Title
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Crystal and molecular structures of the complex of alpha-Chymotrypsin with its inhibitor turkey ovomucoid third domain at 1.8 a resolution.
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Authors
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M.Fujinaga,
A.R.Sielecki,
R.J.Read,
W.Ardelt,
M.Laskowski,
M.N.James.
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Ref.
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J Mol Biol, 1987,
195,
397-418.
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PubMed id
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Secondary reference #2
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Title
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Refined crystal structure of the molecular complex of streptomyces griseus protease b, A serine protease, With the third domain of the ovomucoid inhibitor from turkey.
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Authors
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M.Fujinaga,
R.J.Read,
A.Sielecki,
W.Ardelt,
M.Laskowski,
M.N.James.
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Ref.
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Proc Natl Acad Sci U S A, 1982,
79,
4868-4872.
[DOI no: ]
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PubMed id
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Secondary reference #3
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Title
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Crystal structure studies and inhibition kinetics of tripeptide chloromethyl ketone inhibitors with streptomyces griseus protease b.
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Authors
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M.N.James,
G.D.Brayer,
L.T.Delbaere,
A.R.Sielecki,
A.Gertler.
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Ref.
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J Mol Biol, 1980,
139,
423-438.
[DOI no: ]
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PubMed id
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Figure 2.
RI Phe
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Figure 5.
FIG. 5. Stereo-drawing of the inhibitors: (a) BocGLF and (b) BocAGF bound in the active site
of SGPB, as interpreted from the 2.8 A resolution difference electron density maps of their respec-
tive complexes (Fig. 2). Polypeptide main-chain bonding of the enzyme and all interatomic
bonds of the bound inhibitors are indicated by solid black bonds All oxygen atoms are dis-
tinguished by solid black circles. Hydrogen bnds to active site residues and the boun inhibitors
are indicated by thin broken lines. For the BocGLF-SGPB complex, the new position of the side-
chain of Tyrl71 is shown, while the original native conformation of this residue is indicated by
broken lines.
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The above figures are
reproduced from the cited reference
with permission from Elsevier
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Secondary reference #4
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Title
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The 2.8 a resolution structure of streptomyces griseus protease b and its homology with alpha-Chymotrypsin and streptomyces griseus protease a.
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Authors
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L.T.Delbaere,
G.D.Brayer,
M.N.James.
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Ref.
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Can J Biochem, 1979,
57,
135-144.
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PubMed id
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Secondary reference #5
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Title
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Amino acid sequence alignment of bacterial and mammalian pancreatic serine proteases based on topological equivalences.
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Authors
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M.N.James,
L.T.Delbaere,
G.D.Brayer.
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Ref.
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Can J Biochem, 1978,
56,
396-402.
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PubMed id
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Secondary reference #6
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Title
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Relationship between the structures and activities of some microbial serine proteases. II. Comparison of the tertiary structures of microbial and pancreatic serine proteases
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Author
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M.N.G.James.
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Ref.
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miami winter symp, 1976,
11,
125.
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Secondary reference #7
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Title
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Tertiary structural differences between microbial serine proteases and pancreatic serine enzymes.
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Authors
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L.T.Delbaere,
W.L.Hutcheon,
M.N.James,
W.E.Thiessen.
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Ref.
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Nature, 1975,
257,
758-763.
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PubMed id
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Secondary reference #8
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Title
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The 4.5 angstrom resolution structure of a bacterial serine protease from streptomyces griseus.
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Authors
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P.W.Codding,
L.T.Delbaere,
K.Hayakawa,
W.L.Hutcheon,
M.N.James,
L.Jurásek.
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Ref.
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Can J Biochem, 1974,
52,
208-220.
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PubMed id
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