PDBsum entry 3s7k

Hydrolase

PDB id: 3s7k

Contents

Protein chains

31 a.a.

252 a.a.

33 a.a.

Ligands

TRS ×2

Metals

__K ×2

Waters ×384

PDB id:

3s7k

Name:

Hydrolase

Title:

Structure of thrombin mutant y225p in the e form

Structure:

Prothrombin. Chain: a, c. Fragment: thrombin light chain. Synonym: coagulation factor ii, activation peptide fragment 1, activation peptide fragment 2, thrombin light chain, thrombin heavy chain. Engineered: yes. Mutation: yes. Prothrombin.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: f2. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.90Å R-factor: 0.177 R-free: 0.217

Authors:

W.Niu,Z.Chen,P.Gandhi,A.Vogt,N.Pozzi,L.A.Pele,F.Zapata,E.Di Cera

Key ref:

W.Niu et al. (2011). Crystallographic and kinetic evidence of allostery in a trypsin-like protease. Biochemistry, 50, 6301-6307. PubMed id: 21707111

Date:

26-May-11 Release date: 06-Jul-11

Protein chain

P00734  (THRB_HUMAN) -  Prothrombin from Homo sapiens

Seq: 622 a.a.

Struc: 31 a.a.

Protein chains

P00734  (THRB_HUMAN) -  Prothrombin from Homo sapiens

Seq: 622 a.a.

Struc: 252 a.a.*

Protein chain

P00734  (THRB_HUMAN) -  Prothrombin from Homo sapiens

Seq: 622 a.a.

Struc: 33 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains A, B, C, D: E.C.3.4.21.5 - thrombin.
• Reaction: Preferential cleavage: Arg-|-Gly; activates fibrinogen to fibrin and releases fibrinopeptide A and B.

Biochemistry 50:6301-6307 (2011)

PubMed id: 21707111

Crystallographic and kinetic evidence of allostery in a trypsin-like protease.

W.Niu, Z.Chen, P.S.Gandhi, A.D.Vogt, N.Pozzi, L.A.Pelc, F.Zapata, E.Di Cera.

ABSTRACT

No abstract given.