UniProt functional annotation for P58687

UniProt code: P58687.

Organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
Taxonomy: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Salmonella.
 
Function: Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids. Catalyzes the cis-dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3- dehydroshikimate. The reaction involves the formation of an imine intermediate between the keto group of 3-dehydroquinate and the epsylon-amino group of a lys-170 at the active site. {ECO:0000255|HAMAP-Rule:MF_00214, ECO:0000269|PubMed:21087925, ECO:0000269|PubMed:21291284, ECO:0000269|PubMed:23341204, ECO:0000269|PubMed:24957267}.
 
Catalytic activity: 3-dehydroquinate = 3-dehydroshikimate + H(2)O. {ECO:0000255|HAMAP-Rule:MF_00214, ECO:0000269|PubMed:21087925, ECO:0000269|PubMed:21291284, ECO:0000269|PubMed:23341204, ECO:0000269|PubMed:24957267}.
Enzyme regulation: Inhibited by (2R)-2-methyl-3-dehydroquinic acid. {ECO:0000269|PubMed:24957267}.
Biophysicochemical properties: Kinetic parameters: KM=18 uM for 3-dehydroquinate (at pH 7.2 and 25 degrees Celsius). {ECO:0000269|PubMed:24957267}; KM=21 uM for 3-dehydroquinate (at pH 7.5 and 37 degrees Celsius). {ECO:0000269|PubMed:21087925}; KM=53 uM for 3-dehydroquinate (at pH 7.5 and 37 degrees Celsius). {ECO:0000269|PubMed:23341204}; Note=Kcat is 210 sec(-1) for dehydratase activity with 3- dehydroquinate (at pH 7.5 and 37 degrees Celsius). Kcat is 255 sec(-1) for dehydratase activity with 3-dehydroquinate (at pH 7.2 and 25 degrees Celsius). Kcat is 310 sec(-1) for dehydratase activity with 3-dehydroquinate (at pH 7.5 and 37 degrees Celsius). {ECO:0000269|PubMed:21087925, ECO:0000269|PubMed:21291284, ECO:0000269|PubMed:23341204, ECO:0000269|PubMed:24957267};
Pathway: Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. {ECO:0000255|HAMAP-Rule:MF_00214}.
Subunit: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00214, ECO:0000269|PubMed:21087925, ECO:0000269|PubMed:21291284, ECO:0000269|PubMed:23341204, ECO:0000269|PubMed:24437575, ECO:0000269|Ref.4, ECO:0000269|Ref.7, ECO:0000269|Ref.8}.
Similarity: Belongs to the type-I 3-dehydroquinase family. {ECO:0000255|HAMAP-Rule:MF_00214}.

Annotations taken from UniProtKB at the EBI.