PDBsum entry 3rzw

Protein binding

PDB id: 3rzw

Contents

Protein chains

89 a.a.

75 a.a.

Ligands

GOL ×2

Waters ×138

PDB id:

3rzw

Name:

Protein binding

Title:

Crystal structure of the monobody ysmb-9 bound to human sumo1

Structure:

Monobody ysmb-9. Chain: a, b. Engineered: yes. Small ubiquitin-related modifier 1. Chain: c, d. Synonym: sumo-1, gap-modifying protein 1, gmp1, smt3 homolog 3, sentrin, ubiquitin-homology domain protein pic1, ubiquitin-like protein smt3c, smt3c, ubiquitin-like protein ubl1. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: sumo1, smt3c, smt3h3, ubl1, ok/sw-cl.43. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.15Å R-factor: 0.189 R-free: 0.237

Authors:

R.N.Gilbreth,S.Koide

Key ref:

A.Koide et al. (2012). Teaching an old scaffold new tricks: monobodies constructed using alternative surfaces of the FN3 scaffold. J Mol Biol, 415, 393-405. PubMed id: 22198408

Date:

12-May-11 Release date: 28-Dec-11

Protein chains

No UniProt id for this chain

Struc: 89 a.a.

Protein chains

P63165  (SUMO1_HUMAN) -  Small ubiquitin-related modifier 1 from Homo sapiens

Seq: 101 a.a.

Struc: 75 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains C, D: E.C.?

J Mol Biol 415:393-405 (2012)

PubMed id: 22198408

Teaching an old scaffold new tricks: monobodies constructed using alternative surfaces of the FN3 scaffold.

A.Koide, J.Wojcik, R.N.Gilbreth, R.J.Hoey, S.Koide.

ABSTRACT

No abstract given.