PDBsum entry 3rtr

Ligase

PDB id: 3rtr

Contents

Protein chains

353 a.a.

86 a.a.

317 a.a.

79 a.a.

81 a.a.

81 a.a.

Metals

_ZN ×12

PDB id:

3rtr

Name:

Ligase

Title:

A ring e3-substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-ring ligases

Structure:

Cullin-1. Chain: a, c, e, g. Fragment: unp residues 411-776. Synonym: cul-1. Engineered: yes. Mutation: yes. E3 ubiquitin-protein ligase rbx1. Chain: b, d, f, h. Fragment: unp residues 5-108.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: cul1. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: rbx1, rnf75, roc1. Expression_system_taxid: 562

Resolution:

3.21Å R-factor: 0.238 R-free: 0.283

Authors:

M.F.Calabrese,D.C.Scott,D.M.Duda,C.R.Grace,I.Kurinov,R.W.Kriwacki, B.A.Schulman

Key ref:

M.F.Calabrese et al. (2011). A RING E3-substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases. Nat Struct Biol, 18, 947-949. PubMed id: 21765416

Date:

03-May-11 Release date: 20-Jul-11

Protein chains

Q13616  (CUL1_HUMAN) -  Cullin-1 from Homo sapiens

Seq: 776 a.a.

Struc: 353 a.a.*

Protein chain

P62877  (RBX1_HUMAN) -  E3 ubiquitin-protein ligase RBX1 from Homo sapiens

Seq: 108 a.a.

Struc: 86 a.a.

Protein chain

Q13616  (CUL1_HUMAN) -  Cullin-1 from Homo sapiens

Seq: 776 a.a.

Struc: 317 a.a.*

Protein chain

P62877  (RBX1_HUMAN) -  E3 ubiquitin-protein ligase RBX1 from Homo sapiens

Seq: 108 a.a.

Struc: 79 a.a.

Protein chain

P62877  (RBX1_HUMAN) -  E3 ubiquitin-protein ligase RBX1 from Homo sapiens

Seq: 108 a.a.

Struc: 81 a.a.

Protein chain

P62877  (RBX1_HUMAN) -  E3 ubiquitin-protein ligase RBX1 from Homo sapiens

Seq: 108 a.a.

Struc: 81 a.a.

* PDB and UniProt seqs differ at 8 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chains A, C, E, G: E.C.?
• Enzyme class 3: Chains B, D, F, H: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine
• Enzyme class 4: Chains B, D, F, H: E.C.2.3.2.32 - cullin-RING-type E3 NEDD8 transferase.
• Reaction: S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]- L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N₆-[NEDD8- protein]-yl-[cullin]-L-lysine

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Nat Struct Biol 18:947-949 (2011)

PubMed id: 21765416

A RING E3-substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases.

M.F.Calabrese, D.C.Scott, D.M.Duda, C.R.Grace, I.Kurinov, R.W.Kriwacki, B.A.Schulman.

ABSTRACT

No abstract given.