PDBsum entry 3rrm

Hydrolase

PDB id

3rrm

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Contents

			Protein chains

					375 a.a.


					295 a.a.


					370 a.a.

			Ligands

					ADP


					IHP

			Metals

					_MG

PDB id:

3rrm

Links

Name:

Hydrolase

Title:

S. Cerevisiae dbp5 l327v bound to nup159, gle1 h337r, ip6 and adp

Structure:

Atp-dependent RNA helicase dbp5. Chain: a. Fragment: unp residues 91-482. Synonym: dead box protein 5, helicase ca5/6, ribonucleic acid- trafficking protein 8. Engineered: yes. Mutation: yes. Nucleoporin gle1. Chain: b.

Source:

Saccharomyces cerevisiae. Brewer's yeast,lager beer yeast,yeast. Organism_taxid: 4932. Gene: dbp5, rat8, yor046c. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: brr3, d1049, gle1, rss1, ydl207w. Gene: nup158, nup159, rat7, yil115c.

Resolution:

2.90Å

R-factor:

0.230

R-free:

0.261

Authors:

B.Montpetit,N.D.Thomsen,K.J.Helmke,M.A.Seeliger,J.M.Berger,K.Weis

Key ref:

B.Montpetit et al. (2011). A conserved mechanism of DEAD-box ATPase activation by nucleoporins and InsP6 in mRNA export. Nature, 472, 238-242. PubMed id: 21441902

Date:

29-Apr-11

Release date:

18-May-11

Supersedes:

3pez

PROCHECK

	Headers

	References

Protein chain

P20449 (DBP5_YEAST) - ATP-dependent RNA helicase DBP5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: Struc:					482 a.a. 375 a.a.*

Protein chain

Q12315 (GLE1_YEAST) - mRNA export factor GLE1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: Struc:

Seq: Struc:					538 a.a. 295 a.a.*

Protein chain

P40477 (NU159_YEAST) - Nucleoporin NUP159 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: Struc:

Seq: Struc:

Seq: Struc:					1460 a.a. 370 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 3 residue positions (black crosses)



		Enzyme reactions

Enzyme class 1:

Chain A: E.C.3.6.4.13 - Rna helicase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

ATP + H2O = ADP + phosphate + H⁺

ATP

H2O

ADP
Bound ligand (Het Group name = ADP)
corresponds exactly

phosphate

H(+)

Enzyme class 2:

Chains B, C: E.C.?

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

	Nature 472:238-242 (2011)
PubMed id: 21441902

A conserved mechanism of DEAD-box ATPase activation by nucleoporins and InsP6 in mRNA export.
B.Montpetit, N.D.Thomsen, K.J.Helmke, M.A.Seeliger, J.M.Berger, K.Weis.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

22961380

I.Barbosa, N.Haque, F.Fiorini, C.Barrandon, C.Tomasetto, M.Blanchette, and H.Le Hir (2012).
Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly.

Nat Struct Mol Biol, 19, 983-990.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.