PDBsum entry 3rlf

Hydrolase/transport protein

PDB id

3rlf

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Contents

			Protein chains

					374 a.a.


					490 a.a.


					286 a.a.


					371 a.a.

			Ligands

					GLC-GLC


					UMQ


					PGV ×4


					ANP ×2

			Metals

					_MG ×2

			Waters ×339

PDB id:

3rlf

Links

Name:

Hydrolase/transport protein

Title:

Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to mgamppnp

Structure:

Maltose-binding periplasmic protein. Chain: e. Synonym: mmbp, maltodextrin-binding protein. Engineered: yes. Maltose transport system permease protein malf. Chain: f. Engineered: yes. Maltose transport system permease protein malg. Chain: g.

Source:

Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: male, b4034, jw3994. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: malf, b4033, jw3993. Gene: malg, b4032, jw3992. Gene: malk, b4035, jw3995.

Resolution:

2.20Å

R-factor:

0.225

R-free:

0.254

Authors:

M.L.Oldham,J.Chen

Key ref:

M.L.Oldham and J.Chen (2011). Snapshots of the maltose transporter during ATP hydrolysis. Proc Natl Acad Sci U S A, 108, 15152-15156. PubMed id: 21825153

Date:

19-Apr-11

Release date:

10-Aug-11

PROCHECK

	Headers

	References

Protein chain

P0AEX9 (MALE_ECOLI) - Maltose/maltodextrin-binding periplasmic protein from Escherichia coli (strain K12)

Seq: Struc:					396 a.a. 374 a.a.

Protein chain

P02916 (MALF_ECOLI) - Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12)

Seq: Struc:					514 a.a. 490 a.a.

Protein chain

P68183 (MALG_ECOLI) - Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12)

Seq: Struc:					296 a.a. 286 a.a.

Protein chains

P68187 (MALK_ECOLI) - Maltose/maltodextrin import ATP-binding protein MalK from Escherichia coli (strain K12)

Seq: Struc:					371 a.a. 371 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

Chains A, B: E.C.7.5.2.1 - ABC-type maltose transporter.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

D-maltose(out) + ATP + H2O = D-maltose(in) + ADP + phosphate + H⁺

D-maltose(out)	+	ATP	+	H2O	=	D-maltose(in) Bound ligand (Het Group name = ANP) matches with 81.25% similarity	+	ADP	+	phosphate	+	H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

	Proc Natl Acad Sci U S A 108:15152-15156 (2011)
PubMed id: 21825153

Snapshots of the maltose transporter during ATP hydrolysis.
M.L.Oldham, J.Chen.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

22447242

M.Hohl, C.Briand, M.G.Grütter, and M.A.Seeger (2012).
Crystal structure of a heterodimeric ABC transporter in its inward-facing conformation.

Nat Struct Mol Biol, 19, 395-402.

PDB code:

3qf4

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.