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PDBsum entry 3rje

Go to PDB code: 
protein dna_rna metals links
Transferase, lyase/DNA PDB id
3rje
Jmol PyMol
Contents
Protein chain
329 a.a.
DNA/RNA
Metals
_NA ×4
Waters ×487
PDB id:
3rje
Name: Transferase, lyase/DNA
Title: Ternary complex of DNA polymerase beta with a gapped DNA con 8odg at template position
Structure: DNA polymerase beta. Chain: a. Engineered: yes. DNA (5'-d( Cp Cp Gp Ap Cp (8Og) p Tp Cp Gp Cp Ap Tp Cp Ap Gp C)-3'). Chain: t. Engineered: yes. DNA (5'-d( Gp Cp Tp Gp Ap Tp Gp Cp Gp A)-3'). Chain: p.
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: polb. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic: yes
Resolution:
2.10Å     R-factor:   0.198     R-free:   0.252
Authors: V.K.Batra,W.A.Beard,S.H.Wilson
Key ref: V.K.Batra et al. (2012). Binary complex crystal structure of DNA polymerase β reveals multiple conformations of the templating 8-oxoguanine lesion. Proc Natl Acad Sci U S A, 109, 113-118. PubMed id: 22178760 DOI: 10.1073/pnas.1112235108
Date:
15-Apr-11     Release date:   18-Jan-12    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P06746  (DPOLB_HUMAN) -  DNA polymerase beta
Seq:
Struc:
335 a.a.
329 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.2.7.7.7  - DNA-directed Dna polymerase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Deoxynucleoside triphosphate
+ DNA(n)
= diphosphate
+ DNA(n+1)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     protein complex   6 terms 
  Biological process     immunoglobulin heavy chain V-D-J recombination   27 terms 
  Biochemical function     protein binding     12 terms  

 

 
    reference    
 
 
DOI no: 10.1073/pnas.1112235108 Proc Natl Acad Sci U S A 109:113-118 (2012)
PubMed id: 22178760  
 
 
Binary complex crystal structure of DNA polymerase β reveals multiple conformations of the templating 8-oxoguanine lesion.
V.K.Batra, D.D.Shock, W.A.Beard, C.E.McKenna, S.H.Wilson.
 
  ABSTRACT  
 
No abstract given.

 

 

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