PDBsum entry 3rfs

Protein binding

PDB id: 3rfs

Contents

Protein chains

263 a.a.

Metals

_MG ×2

Waters ×218

PDB id:

3rfs

Name:

Protein binding

Title:

Design of a binding scaffold based on variable lymphocyte receptors of jawless vertebrates by module engineering

Structure:

Internalin b, repeat modules, variable lymphocyte receptor b. Chain: a, b. Engineered: yes. Other_details: chimera of internalin b, repeat modules, variable lymphocyte receptor b

Source:

Listeria monocytogenes, eptatretus burgeri, synthetic. Inshore hagfish. Organism_taxid: 637381, 7764, 32630. Strain: 08-5923. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.70Å R-factor: 0.199 R-free: 0.242

Authors:

H.J.Kim,H.K.Cheong,Y.H.Jeon

Key ref:

S.C.Lee et al. (2012). Design of a binding scaffold based on variable lymphocyte receptors of jawless vertebrates by module engineering. Proc Natl Acad Sci U S A, 109, 3299-3304. PubMed id: 22328160

Date:

06-Apr-11 Release date: 14-Mar-12

Protein chains

Q4G1L3  (Q4G1L3_EPTBU) -  Variable lymphocyte receptor B from Eptatretus burgeri

Seq: 305 a.a.

Struc: 263 a.a.*

* PDB and UniProt seqs differ at 78 residue positions (black crosses)

Proc Natl Acad Sci U S A 109:3299-3304 (2012)

PubMed id: 22328160

Design of a binding scaffold based on variable lymphocyte receptors of jawless vertebrates by module engineering.

S.C.Lee, K.Park, J.Han, J.J.Lee, H.J.Kim, S.Hong, W.Heu, Y.J.Kim, J.S.Ha, S.G.Lee, H.K.Cheong, Y.H.Jeon, D.Kim, H.S.Kim.

ABSTRACT

No abstract given.