PDBsum entry 3qlp

Hydrolase/hydrolase inhibitor/DNA

PDB id: 3qlp

Contents

Protein chains

259 a.a.

34 a.a.

DNA/RNA

Ligands

0G6

NAG

Metals

_NA

__K

Waters ×252

PDB id:

3qlp

Name:

Hydrolase/hydrolase inhibitor/DNA

Title:

X-ray structure of the complex between human alpha thrombin and a modified thrombin binding aptamer (mtba)

Structure:

Thrombin heavy chain. Chain: h. Engineered: yes. Thrombin light chain. Chain: l. Engineered: yes. Modified thrombin binding DNA aptamer. Chain: d. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: f2. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes

Resolution:

2.14Å R-factor: 0.183 R-free: 0.237

Authors:

I.Russo Krauss,A.Merlino,L.Mazzarella,F.Sica

Key ref:

I.Russo Krauss et al. (2011). Thrombin-aptamer recognition: a revealed ambiguity. Nucleic Acids Res, 39, 7858-7867. PubMed id: 21715374

Date:

03-Feb-11 Release date: 19-Oct-11

Protein chain

P00734  (THRB_HUMAN) -  Prothrombin from Homo sapiens

Seq: 622 a.a.

Struc: 259 a.a.

Protein chain

P00734  (THRB_HUMAN) -  Prothrombin from Homo sapiens

Seq: 622 a.a.

Struc: 34 a.a.

DNA/RNA chain

G-G-T-T-G-G-T-G-T-G-G-T-T-G-G 15 bases

Enzyme reactions

• Enzyme class: Chains H, L: E.C.3.4.21.5 - thrombin.
• Reaction: Preferential cleavage: Arg-|-Gly; activates fibrinogen to fibrin and releases fibrinopeptide A and B.

Nucleic Acids Res 39:7858-7867 (2011)

PubMed id: 21715374

Thrombin-aptamer recognition: a revealed ambiguity.

I.Russo Krauss, A.Merlino, C.Giancola, A.Randazzo, L.Mazzarella, F.Sica.

ABSTRACT

No abstract given.