PDBsum entry 3qkq

Hydrolase

PDB id: 3qkq

Contents

Protein chain

296 a.a.

Ligands

VO4

TRS

GOL ×3

Waters ×276

PDB id:

3qkq

Name:

Hydrolase

Title:

Protein tyrosine phosphatase 1b - w179f mutant bound with vanadate

Structure:

Tyrosine-protein phosphatase non-receptor type 1. Chain: a. Fragment: catalytic domain, residues 1-321. Synonym: protein-tyrosine phosphatase 1b, ptp-1b. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ptp1b, ptpn1. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.20Å R-factor: 0.199 R-free: 0.254

Authors:

T.A.S.Brandao,S.J.Johnson,A.C.Hengge

Key ref:

T.A.Brandão et al. (2012). The molecular details of WPD-loop movement differ in the protein-tyrosine phosphatases YopH and PTP1B. Arch Biochem Biophys, 525, 53-59. PubMed id: 22698963

Date:

01-Feb-11 Release date: 08-Aug-12

Protein chain

P18031  (PTN1_HUMAN) -  Tyrosine-protein phosphatase non-receptor type 1 from Homo sapiens

Seq: 435 a.a.

Struc: 296 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.1.3.48 - protein-tyrosine-phosphatase.
• Reaction: O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

Arch Biochem Biophys 525:53-59 (2012)

PubMed id: 22698963

The molecular details of WPD-loop movement differ in the protein-tyrosine phosphatases YopH and PTP1B.

T.A.Brandão, S.J.Johnson, A.C.Hengge.

ABSTRACT

No abstract given.