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PDBsum entry 3qhr
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protein ligands metals Protein-protein interface(s) links
Transferase/protein binding PDB id
3qhr

 

 

 

 

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Contents
Protein chains
298 a.a.
261 a.a.
Ligands
PRO-LYS-THR-PRO-
LYS-LYS-ALA-LYS-
LYS-LEU ×4
ADP ×2
MGF ×2
GOL ×17
Metals
_MG ×4
_CL ×2
Waters ×601
PDB id:
3qhr
Name: Transferase/protein binding
Title: Structure of a pcdk2/cyclina transition-state mimic
Structure: Cell division protein kinase 2. Chain: a, c. Synonym: p33 protein kinase. Engineered: yes. Cyclin-a2. Chain: b, d. Fragment: unp residues 163-422. Synonym: cyclin-a. Engineered: yes.
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: cdk2. Expressed in: escherichia coli. Expression_system_taxid: 562. Mus musculus. Mouse. Organism_taxid: 10090.
Resolution:
2.17Å     R-factor:   0.181     R-free:   0.210
Authors: M.A.Young,D.M.Jacobsen,Z.Q.Bao
Key ref: Z.Q.Bao et al. (2011). Briefly bound to activate: transient binding of a second catalytic magnesium activates the structure and dynamics of CDK2 kinase for catalysis. Structure, 19, 675-690. PubMed id: 21565702
Date:
26-Jan-11     Release date:   25-May-11    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P24941  (CDK2_HUMAN) -  Cyclin-dependent kinase 2 from Homo sapiens
Seq:
Struc: 		298 a.a.
298 a.a.*
Protein chains
Pfam   ArchSchema ?
P51943  (CCNA2_MOUSE) -  Cyclin-A2 from Mus musculus
Seq:
Struc: 		422 a.a.
261 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chains A, C: E.C.2.7.11.22  - cyclin-dependent kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction:
1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H+
2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H+
L-seryl-[protein]
 + ATP
 = O-phospho-L-seryl-[protein]
Bound ligand (Het Group name = ADP)
corresponds exactly 		+ ADP
 + H(+)
L-threonyl-[protein]
 + ATP
 = O-phospho-L-threonyl-[protein]
Bound ligand (Het Group name = ADP)
corresponds exactly 		+ ADP
 + H(+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
Structure 19:675-690 (2011)
PubMed id: 21565702  
 
 
Briefly bound to activate: transient binding of a second catalytic magnesium activates the structure and dynamics of CDK2 kinase for catalysis.
Z.Q.Bao, D.M.Jacobsen, M.A.Young.
 
  ABSTRACT  
 
No abstract given.

 

 

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