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PDBsum entry 3q8m

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protein dna_rna metals Protein-protein interface(s) links
Hydrolase/DNA PDB id
3q8m
Jmol PyMol
Contents
Protein chain
335 a.a.
DNA/RNA
Metals
__K ×2
Waters ×110
PDB id:
3q8m
Name: Hydrolase/DNA
Title: Crystal structure of human flap endonuclease fen1 (d181a) in with substrate 5'-flap DNA and k+
Structure: Flap endonuclease 1. Chain: a, b. Fragment: d181a. Synonym: fen-1, dnase iv, flap structure-specific endonucle maturation factor 1, mf1, hfen-1. Engineered: yes. DNA (5'- d( Ap Cp Tp Cp Tp Gp Cp Cp Tp Cp Ap Ap Gp Ap Cp Gp Gp T)-3' chain: d, g.
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: fen1, rad2. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Other_details: synthesized. Other_details: synthesized
Resolution:
2.60Å     R-factor:   0.220     R-free:   0.272
Authors: S.E.Tsutakawa,S.Classen,B.R.Chapados,A.Arvai,D.L.Finger,G.Gu C.G.Tomlinson,P.Thompson,A.H.Sarker,B.Shen,P.K.Cooper,J.A.G J.A.Tainer
Key ref: S.E.Tsutakawa et al. (2011). Human flap endonuclease structures, DNA double-base flipping, and a unified understanding of the FEN1 superfamily. Cell, 145, 198-211. PubMed id: 21496641
Date:
06-Jan-11     Release date:   27-Apr-11    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P39748  (FEN1_HUMAN) -  Flap endonuclease 1
Seq:
Struc:
380 a.a.
335 a.a.*
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.3.1.-.-
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     DNA repair   1 term 
  Biochemical function     catalytic activity     4 terms  

 

 
Cell 145:198-211 (2011)
PubMed id: 21496641  
 
 
Human flap endonuclease structures, DNA double-base flipping, and a unified understanding of the FEN1 superfamily.
S.E.Tsutakawa, S.Classen, B.R.Chapados, A.S.Arvai, L.D.Finger, G.Guenther, C.G.Tomlinson, P.Thompson, A.H.Sarker, B.Shen, P.K.Cooper, J.A.Grasby, J.A.Tainer.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
23396808 S.Classen, G.L.Hura, J.M.Holton, R.P.Rambo, I.Rodic, P.J.McGuire, K.Dyer, M.Hammel, G.Meigs, K.A.Frankel, and J.A.Tainer (2013).
Implementation and performance of SIBYLS: a dual endstation small-angle X-ray scattering and macromolecular crystallography beamline at the Advanced Light Source.
  J Appl Crystallogr, 46, 1.  
23104058 K.Shi, K.Kurahashi, R.Gao, S.E.Tsutakawa, J.A.Tainer, Y.Pommier, and H.Aihara (2012).
Structural basis for recognition of 5'-phosphotyrosine adducts by Tdp2.
  Nat Struct Mol Biol, 19, 1372-1377.
PDB codes: 4f1h 4f1i 4fpv 4fva 4gew
23104055 M.J.Schellenberg, C.D.Appel, S.Adhikari, P.D.Robertson, D.A.Ramsden, and R.S.Williams (2012).
Mechanism of repair of 5'-topoisomerase II-DNA adducts by mammalian tyrosyl-DNA phosphodiesterase 2.
  Nat Struct Mol Biol, 19, 1363-1371.
PDB codes: 4gyz 4gz0 4gz1 4gz2
21496637 R.S.Williams, and T.A.Kunkel (2011).
FEN nucleases: bind, bend, fray, cut.
  Cell, 145, 171-172.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.

 

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