PDBsum entry 3q8m

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protein dna_rna metals Protein-protein interface(s) links
Hydrolase/DNA PDB id
Jmol PyMol
Protein chain
335 a.a.
__K ×2
Waters ×110
PDB id:
Name: Hydrolase/DNA
Title: Crystal structure of human flap endonuclease fen1 (d181a) in with substrate 5'-flap DNA and k+
Structure: Flap endonuclease 1. Chain: a, b. Fragment: d181a. Synonym: fen-1, dnase iv, flap structure-specific endonucle maturation factor 1, mf1, hfen-1. Engineered: yes. DNA (5'- d( Ap Cp Tp Cp Tp Gp Cp Cp Tp Cp Ap Ap Gp Ap Cp Gp Gp T)-3' chain: d, g.
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: fen1, rad2. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Other_details: synthesized. Other_details: synthesized
2.60Å     R-factor:   0.220     R-free:   0.272
Authors: S.E.Tsutakawa,S.Classen,B.R.Chapados,A.Arvai,D.L.Finger,G.Gu C.G.Tomlinson,P.Thompson,A.H.Sarker,B.Shen,P.K.Cooper,J.A.G J.A.Tainer
Key ref: S.E.Tsutakawa et al. (2011). Human flap endonuclease structures, DNA double-base flipping, and a unified understanding of the FEN1 superfamily. Cell, 145, 198-211. PubMed id: 21496641
06-Jan-11     Release date:   27-Apr-11    
Go to PROCHECK summary

Protein chains
Pfam   ArchSchema ?
P39748  (FEN1_HUMAN) -  Flap endonuclease 1
380 a.a.
335 a.a.*
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.3.1.-.-
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     DNA repair   1 term 
  Biochemical function     catalytic activity     4 terms  


Cell 145:198-211 (2011)
PubMed id: 21496641  
Human flap endonuclease structures, DNA double-base flipping, and a unified understanding of the FEN1 superfamily.
S.E.Tsutakawa, S.Classen, B.R.Chapados, A.S.Arvai, L.D.Finger, G.Guenther, C.G.Tomlinson, P.Thompson, A.H.Sarker, B.Shen, P.K.Cooper, J.A.Grasby, J.A.Tainer.
No abstract given.


Literature references that cite this PDB file's key reference

  PubMed id Reference
23396808 S.Classen, G.L.Hura, J.M.Holton, R.P.Rambo, I.Rodic, P.J.McGuire, K.Dyer, M.Hammel, G.Meigs, K.A.Frankel, and J.A.Tainer (2013).
Implementation and performance of SIBYLS: a dual endstation small-angle X-ray scattering and macromolecular crystallography beamline at the Advanced Light Source.
  J Appl Crystallogr, 46, 1.  
23104058 K.Shi, K.Kurahashi, R.Gao, S.E.Tsutakawa, J.A.Tainer, Y.Pommier, and H.Aihara (2012).
Structural basis for recognition of 5'-phosphotyrosine adducts by Tdp2.
  Nat Struct Mol Biol, 19, 1372-1377.
PDB codes: 4f1h 4f1i 4fpv 4fva 4gew
23104055 M.J.Schellenberg, C.D.Appel, S.Adhikari, P.D.Robertson, D.A.Ramsden, and R.S.Williams (2012).
Mechanism of repair of 5'-topoisomerase II-DNA adducts by mammalian tyrosyl-DNA phosphodiesterase 2.
  Nat Struct Mol Biol, 19, 1363-1371.
PDB codes: 4gyz 4gz0 4gz1 4gz2
21496637 R.S.Williams, and T.A.Kunkel (2011).
FEN nucleases: bind, bend, fray, cut.
  Cell, 145, 171-172.  
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