PDBsum entry 3q47

Ligase/transcription

PDB id: 3q47

Contents

Protein chain

132 a.a.

Ligands

ASN-PRO-ILE-SER-ASP-VAL-ASP

Waters ×166

PDB id:

3q47

Name:

Ligase/transcription

Title:

Crystal structure of tpr domain of chip complexed with pseudophosphorylated smad1 peptide

Structure:

Stip1 homology and u box-containing protein 1. Chain: b. Fragment: tpr domain. Synonym: carboxy terminus of hsp70-interacting protein, e3 ubiquitin- protein ligase chip. Engineered: yes. Smad1 peptide. Chain: c. Engineered: yes.

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: stub1, chip. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Homo sapiens. Human.

Resolution:

1.71Å R-factor: 0.174 R-free: 0.192

Authors:

L.Wang,L.Chen,J.W.Wu

Key ref:

L.Wang et al. (2011). Molecular mechanism of the negative regulation of Smad1/5 protein by carboxyl terminus of Hsc70-interacting protein (CHIP). J Biol Chem, 286, 15883-15894. PubMed id: 21454478

Date:

23-Dec-10 Release date: 16-Mar-11

Protein chain

Q9WUD1  (CHIP_MOUSE) -  E3 ubiquitin-protein ligase CHIP from Mus musculus

Seq: 304 a.a.

Struc: 132 a.a.

Enzyme reactions

• Enzyme class: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine

J Biol Chem 286:15883-15894 (2011)

PubMed id: 21454478

Molecular mechanism of the negative regulation of Smad1/5 protein by carboxyl terminus of Hsc70-interacting protein (CHIP).

L.Wang, Y.T.Liu, R.Hao, L.Chen, Z.Chang, H.R.Wang, Z.X.Wang, J.W.Wu.

ABSTRACT

No abstract given.