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PDBsum entry 3pux
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protein ligands metals Protein-protein interface(s) links
Hydrolase/transport protein PDB id
3pux

 

 

 

 

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Contents
Protein chains
374 a.a.
490 a.a.
293 a.a.
371 a.a.
Ligands
GLC-GLC
UMQ
PGV ×10
ADP-BEF ×2
Metals
_MG ×2
Waters ×252
PDB id:
3pux
Name: Hydrolase/transport protein
Title: Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3
Structure: Maltose-binding periplasmic protein. Chain: e. Fragment: unp residues 27-396. Synonym: mmbp, maltodextrin-binding protein. Engineered: yes. Maltose transport system permease protein malf. Chain: f. Engineered: yes. Maltose transport system permease protein malg.
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: b4034, ecdh10b_4223, jw3994, male. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: b4033, ecdh10b_4222, jw3993, malf. Gene: b4032, ecdh10b_4221, jw3992, malg. Gene: b4035, ecdh10b_4224, jw3995, malk.
Resolution:
2.30Å     R-factor:   0.230     R-free:   0.265
Authors: M.L.Oldham,J.Chen
Key ref: M.L.Oldham and J.Chen (2011). Snapshots of the maltose transporter during ATP hydrolysis. Proc Natl Acad Sci U S A, 108, 15152-15156. PubMed id: 21825153
Date:
06-Dec-10     Release date:   10-Aug-11    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P0AEX9  (MALE_ECOLI) -  Maltose/maltodextrin-binding periplasmic protein from Escherichia coli (strain K12)
Seq:
Struc: 		396 a.a.
374 a.a.
Protein chain
Pfam   ArchSchema ?
P02916  (MALF_ECOLI) -  Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12)
Seq:
Struc: 		514 a.a.
490 a.a.
Protein chain
Pfam   ArchSchema ?
P68183  (MALG_ECOLI) -  Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12)
Seq:
Struc: 		296 a.a.
293 a.a.
Protein chains
Pfam   ArchSchema ?
P68187  (MALK_ECOLI) -  Maltose/maltodextrin import ATP-binding protein MalK from Escherichia coli (strain K12)
Seq:
Struc: 		371 a.a.
371 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: Chains A, B: E.C.7.5.2.1  - ABC-type maltose transporter.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: D-maltose(out) + ATP + H2O = D-maltose(in) + ADP + phosphate + H+
D-maltose(out)
 + ATP
 + H2O
 = D-maltose(in)
Bound ligand (Het Group name = ADP)
corresponds exactly 		+ ADP
 + phosphate
 + H(+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
Proc Natl Acad Sci U S A 108:15152-15156 (2011)
PubMed id: 21825153  
 
 
Snapshots of the maltose transporter during ATP hydrolysis.
M.L.Oldham, J.Chen.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
22447242 M.Hohl, C.Briand, M.G.Grütter, and M.A.Seeger (2012).
Crystal structure of a heterodimeric ABC transporter in its inward-facing conformation.
  Nat Struct Mol Biol, 19, 395-402.
PDB code: 3qf4
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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