UniProt functional annotation for P40422

UniProt code: P40422.

Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Taxonomy: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
 
Function: DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively. RNA polymerases are composed of mobile elements that move relative to each other. In Pol II, the core element with the central large cleft comprises RPB3, RBP10, RPB11, RPB12 and regions of RPB1 and RPB2 forming the active center. {ECO:0000269|PubMed:18160037, ECO:0000269|PubMed:24153182, ECO:0000269|PubMed:24153184}.
 
Subunit: Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes. Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits: RPA135, RPA190, RPC40, RPA14, RPB5, RPO26, RPA43, RPB8, RPA12, RPB10, RPC19, RPC10, RPA49 and RPA34. The complex is composed of a horseshoe-shaped core containing ten subunits (RPA135, RPA190, RPB5, RPO26, RPB8, RPB10, RPC10, RPA12, RPC19 and RPC40) where RPA135 and RPA190 form the DNA-binding cleft. Outside of the core, RPA14 and RPA43 form the stalk that mediates interactions with transcription initiation factors and newly synthesized RNA. Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits: RPO21, RPB2, RPB3, RPB4, RPB5, RPO26, RPB7, RPB8, RPB9, RPB10 and RPC10. Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits. Interacts, via its C- terminus, with TFIIIC subunit TFC4. {ECO:0000269|PubMed:10559229, ECO:0000269|PubMed:11805306, ECO:0000269|PubMed:12914699, ECO:0000269|PubMed:16341226, ECO:0000269|PubMed:18160037, ECO:0000269|PubMed:24153182, ECO:0000269|PubMed:24153184}.
Subcellular location: Nucleus, nucleolus {ECO:0000269|PubMed:14562095}.
Ptm: The N-terminus is blocked.
Miscellaneous: Present with 907 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Similarity: Belongs to the archaeal RpoP/eukaryotic RPC10 RNA polymerase subunit family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.