 |
PDBsum entry 3pn7
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Protein binding
|
PDB id
|
|
|
|
3pn7
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
|
|
|
68 a.a.
|
|
|
|
|
|
|
|
|
|
|
141 a.a.
|
|
|
|
|
|
|
|
|
|
|
152 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
References listed in PDB file
|
|
|
Key reference
|
|
|
Title
|
|
Visualizing key hinges and a potential major source of compliance in the lever arm of myosin.
|
|
|
Authors
|
|
J.H.Brown,
V.S.Kumar,
E.O'Neall-Hennessey,
L.Reshetnikova,
H.Robinson,
M.Nguyen-Mccarty,
A.G.Szent-Györgyi,
C.Cohen.
|
|
|
Ref.
|
|
Proc Natl Acad Sci U S A, 2011,
108,
114-119.
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
We have determined the 2.3-Å-resolution crystal structure of a myosin light
chain domain, corresponding to one type found in sea scallop catch
("smooth") muscle. This structure reveals hinges that may function in
the "on" and "off" states of myosin. The molecule adopts two
different conformations about the heavy chain "hook" and regulatory
light chain (RLC) helix D. This conformational change results in extended and
compressed forms of the lever arm whose lengths differ by 10 Å. The heavy chain
hook and RLC helix D hinges could thus serve as a potential major and localized
source of cross-bridge compliance during the contractile cycle. In addition, in
one of the molecules of the crystal, part of the RLC N-terminal extension is
seen in atomic detail and forms a one-turn alpha-helix that interacts with RLC
helix D. This extension, whose sequence is highly variable in different myosins,
may thus modulate the flexibility of the lever arm. Moreover, the relative
proximity of the phosphorylation site to the helix D hinge suggests a potential
role for conformational changes about this hinge in the transition between the
on and off states of regulated myosins.
|
|
|
|
|
|
|
