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PDBsum entry 3pn7
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Protein binding
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PDB id
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3pn7
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Contents |
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68 a.a.
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141 a.a.
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152 a.a.
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PDB id:
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Protein binding
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Title:
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Visualizing new hinges and a potential major source of compliance in the lever arm of myosin
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Structure:
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Myosin heavy chain. Chain: a, d. Myosin regulatory light chain. Chain: b, e. Myosin essential light chain. Chain: c, f
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Source:
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Placopecten magellanicus. Sea scallop. Organism_taxid: 6577. Other_details: muscle. Other_details: muscle
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Resolution:
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2.25Å
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R-factor:
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0.191
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R-free:
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0.241
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Authors:
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J.H.Brown,V.S.Senthil-Kumar,E.O'Neall-Hennessey,L.Reshetnikova, H.Robinson,M.Nguyen-Mccarty,A.G.Szent-Gyorgyi,C.Cohen
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Key ref:
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J.H.Brown
et al.
(2011).
Visualizing key hinges and a potential major source of compliance in the lever arm of myosin.
Proc Natl Acad Sci U S A,
108,
114-119.
PubMed id:
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Date:
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18-Nov-10
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Release date:
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05-Jan-11
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PROCHECK
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Headers
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References
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Q26080
(Q26080_PLAMG) -
Myosin heavy chain from Placopecten magellanicus
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Seq:
Struc:
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1950 a.a.
68 a.a.
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Proc Natl Acad Sci U S A
108:114-119
(2011)
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PubMed id:
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Visualizing key hinges and a potential major source of compliance in the lever arm of myosin.
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J.H.Brown,
V.S.Kumar,
E.O'Neall-Hennessey,
L.Reshetnikova,
H.Robinson,
M.Nguyen-McCarty,
A.G.Szent-Györgyi,
C.Cohen.
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ABSTRACT
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We have determined the 2.3-Å-resolution crystal structure of a myosin light
chain domain, corresponding to one type found in sea scallop catch
("smooth") muscle. This structure reveals hinges that may function in
the "on" and "off" states of myosin. The molecule adopts two
different conformations about the heavy chain "hook" and regulatory
light chain (RLC) helix D. This conformational change results in extended and
compressed forms of the lever arm whose lengths differ by 10 Å. The heavy chain
hook and RLC helix D hinges could thus serve as a potential major and localized
source of cross-bridge compliance during the contractile cycle. In addition, in
one of the molecules of the crystal, part of the RLC N-terminal extension is
seen in atomic detail and forms a one-turn alpha-helix that interacts with RLC
helix D. This extension, whose sequence is highly variable in different myosins,
may thus modulate the flexibility of the lever arm. Moreover, the relative
proximity of the phosphorylation site to the helix D hinge suggests a potential
role for conformational changes about this hinge in the transition between the
on and off states of regulated myosins.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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O.Pylypenko,
and
A.M.Houdusse
(2011).
Essential "ankle" in the myosin lever arm.
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Proc Natl Acad Sci U S A,
108,
5-6.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
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Links
