PDBsum entry 3pls

Transferase

PDB id: 3pls

Contents

Protein chain

298 a.a. *

Ligands

ANP

Metals

_MG

Waters ×50

* Residue conservation analysis

PDB id:

3pls

Name:

Transferase

Title:

Ron in complex with ligand amp-pnp

Structure:

Macrophage-stimulating protein receptor. Chain: a. Fragment: ron kinase domain residues 1060-1357. Synonym: msp receptor, cdw136, protein-tyrosine kinase 8, p185-ron, macrophage-stimulating protein receptor beta chain. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mst1r, ptk8, ron. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108.

Resolution:

2.24Å R-factor: 0.235 R-free: 0.290

Authors:

J.Wang,S.Steinbacher,M.Augustin,P.Schreiner,D.Epstein,M.J.Mulvihill, A.P.Crew

Key ref:

J.Wang et al. (2010). The crystal structure of a constitutively active mutant RON kinase suggests an intramolecular autophosphorylation hypothesis. Biochemistry, 49, 7972-7974. PubMed id: 20726546

Date:

15-Nov-10 Release date: 24-Nov-10

Protein chain

Q04912  (RON_HUMAN) -  Macrophage-stimulating protein receptor from Homo sapiens

Seq: 1400 a.a.

Struc: 298 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.7.10.1 - receptor protein-tyrosine kinase.
• Reaction: L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H⁺

L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] (Bound ligand (Het Group name = ANP) matches with 81.25% similarity) + ADP + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

Biochemistry 49:7972-7974 (2010)

PubMed id: 20726546

The crystal structure of a constitutively active mutant RON kinase suggests an intramolecular autophosphorylation hypothesis.

J.Wang, S.Steinbacher, M.Augustin, P.Schreiner, D.Epstein, M.J.Mulvihill, A.P.Crew.

ABSTRACT

No abstract given.