PDBsum entry 3pfv

Ligase/protein binding

PDB id: 3pfv

Contents

Protein chains

302 a.a. *

11 a.a. *

Ligands

LEU-GLN-ARG-PTR-SER-SER-ASP-PRO-THR-GLY

SO4 ×7

EDO ×3

PG4

Metals

_NA ×2

_CL ×2

Waters ×220

* Residue conservation analysis

PDB id:

3pfv

Name:

Ligase/protein binding

Title:

Crystal structure of cbl-b tkb domain in complex with egfr py1069 peptide

Structure:

E3 ubiquitin-protein ligase cbl-b. Chain: a, b. Fragment: n-terminal tkb domain (unp residues 38-344). Synonym: casitas b-lineage lymphoma proto-oncogene b, ring finger protein 56, sh3-binding protein cbl-b, signal transduction protein cbl-b. Engineered: yes. 11-meric peptide from epidermal growth factor receptor. Chain: c, d.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: cblb, nbla00127, rnf56. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Organism_taxid: 9606

Resolution:

2.27Å R-factor: 0.219 R-free: 0.262

Authors:

A.Chaikuad,K.Guo,C.D.O.Cooper,V.Ayinampudi,T.Krojer,J.R.C.Muniz, M.Vollmar,P.Canning,O.Gileadi,F.Von Delft,C.H.Arrowsmith,J.Weigelt, A.M.Edwards,C.Bountra,A.Bullock,Structural Genomics Consortium (Sgc)

Key ref:

A.Chaikuad et al. Crystal structure of cbl-B tkb domain in complex with py1069 peptide. To be published, .

Date:

29-Oct-10 Release date: 08-Dec-10

Protein chains

Q13191  (CBLB_HUMAN) -  E3 ubiquitin-protein ligase CBL-B from Homo sapiens

Seq: 982 a.a.

Struc: 302 a.a.*

Protein chain

P00533  (EGFR_HUMAN) -  Epidermal growth factor receptor from Homo sapiens

Seq: 1210 a.a.

Struc: 11 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: Chains A, B: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine
• Enzyme class 3: Chain D: E.C.2.7.10.1 - receptor protein-tyrosine kinase.
• Reaction: L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site