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PDBsum entry 3pex

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protein ligands Protein-protein interface(s) links
Hydrolase PDB id
3pex
Jmol
Contents
Protein chains
389 a.a.
293 a.a.
Ligands
ADP
IHP ×3
Superseded by: 3rrn
PDB id:
3pex
Name: Hydrolase
Title: S. Cerevisiae dbp5 l327v bound to gle1 h337r and ip6
Structure: Atp-dependent RNA helicase dbp5. Chain: a. Synonym: dead box protein 5, helicase ca5/6, ribonucleic ac trafficking protein 8. Engineered: yes. Mutation: yes. Nucleoporin gle1. Chain: b. Synonym: nuclear pore protein gle1, RNA export factor gle1.
Source: Saccharomyces cerevisiae. Yeast. Organism_taxid: 4932. Gene: dbp5, rat8, yor046c. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: brr3, d1049, gle1, rss1, ydl207w.
Resolution:
4.00Å     R-factor:   0.216     R-free:   0.239
Authors: B.Montpetit,N.D.Thomsen,K.J.Helmke,M.A.Seeliger,J.M.Berger,K
Key ref: B.Montpetit et al. (2011). A conserved mechanism of DEAD-box ATPase activation by nucleoporins and InsP6 in mRNA export. Nature, 472, 238-242. PubMed id: 21441902
Date:
27-Oct-10     Release date:   23-Mar-11    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P20449  (DBP5_YEAST) -  ATP-dependent RNA helicase DBP5
Seq:
Struc:
482 a.a.
389 a.a.*
Protein chain
Pfam   ArchSchema ?
Q12315  (GLE1_YEAST) -  Nucleoporin GLE1
Seq:
Struc:
 
Seq:
Struc:
538 a.a.
293 a.a.*
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 5 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chain A: E.C.3.6.4.13  - Rna helicase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + H2O = ADP + phosphate
ATP
+ H(2)O
=
ADP
Bound ligand (Het Group name = ADP)
corresponds exactly
+ phosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
Nature 472:238-242 (2011)
PubMed id: 21441902  
 
 
A conserved mechanism of DEAD-box ATPase activation by nucleoporins and InsP6 in mRNA export.
B.Montpetit, N.D.Thomsen, K.J.Helmke, M.A.Seeliger, J.M.Berger, K.Weis.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
22961380 I.Barbosa, N.Haque, F.Fiorini, C.Barrandon, C.Tomasetto, M.Blanchette, and H.Le Hir (2012).
Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly.
  Nat Struct Mol Biol, 19, 983-990.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.