UniProt functional annotation for P22734

UniProt code: P22734.

Organism: Rattus norvegicus (Rat).
Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Function: Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
Catalytic activity: S-adenosyl-L-methionine + a catechol = S- adenosyl-L-homocysteine + a guaiacol.
Cofactor: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;
Biophysicochemical properties: Kinetic parameters: KM=30 uM for S-adenosyl-L-methionine {ECO:0000269|PubMed:16618795}; Vmax=377 nmol/h/mg enzyme {ECO:0000269|PubMed:16618795};
Subcellular location: Isoform 2: Cytoplasm.
Subcellular location: Isoform 1: Cell membrane; Single-pass type II membrane protein; Extracellular side.
Ptm: The N-terminus is blocked.
Similarity: Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O- methyltransferase family. {ECO:0000255|PROSITE-ProRule:PRU01019}.
Sequence caution: Sequence=AAA40881.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=AAA40882.1; Type=Erroneous initiation; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.