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PDBsum entry 3ons
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Signaling protein
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PDB id
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3ons
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References listed in PDB file
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Key reference
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Title
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The structure of human ubiquitin in 2-Methyl-2,4-Pentanediol: a new conformational switch.
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Authors
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K.Y.Huang,
G.A.Amodeo,
L.Tong,
A.Mcdermott.
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Ref.
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Protein Sci, 2011,
20,
630-639.
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PubMed id
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Abstract
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A new crystal structure of human ubiquitin is reported at 1.8 Å resolution.
Compared with the other known crystal structure or the solution NMR structure of
monomeric human ubiquitin, this new structure is similar in its overall fold but
differs with respect to the conformation of the backbone in a surface-exposed
region. The conformation reported here resembles conformations previously seen
in complex with deubiquinating enzymes, wherein the Asp52/Gly53 main chain and
Glu24 side chain move. This movement exposes the backbone carbonyl of Asp52 to
the exterior of the molecule, making it possible to engage in hydrogen-bond
contacts with neighboring molecules, rather than in an internal hydrogen bond
with the backbone of Glu24. This particular crystal form of ubiquitin has been
used in a large number of solid state NMR studies. The structure described here
elucidates the origin of many of the chemical shift differences comparing
solution and solid state studies.
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