PDBsum entry 3om2

Transferase

PDB id: 3om2

Contents

Protein chain

448 a.a.

Ligands

CIT ×2

Metals

_MG ×2

_CA

Waters ×657

PDB id:

3om2

Name:

Transferase

Title:

Crystal structure of b. Megaterium levansucrase mutant d257a

Structure:

Levansucrase. Chain: a. Fragment: levansucrase sacb, unp residues 29-484. Engineered: yes. Mutation: yes

Source:

Bacillus megaterium. Bacillus fructosus. Organism_taxid: 1404. Gene: sacb. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.90Å R-factor: 0.163 R-free: 0.218

Authors:

C.P.Strube,A.Homann,M.Gamer,D.Jahn,J.Seibel,D.W.Heinz

Key ref:

C.P.Strube et al. (2011). Polysaccharide synthesis of the levansucrase SacB from Bacillus megaterium is controlled by distinct surface motifs. J Biol Chem, 286, 17593-17600. PubMed id: 21454585

Date:

26-Aug-10 Release date: 23-Mar-11

Protein chain

D5DC07  (D5DC07_PRIM3) -  Levansucrase from Priestia megaterium (strain DSM 319 / IMG 1521)

Seq: 484 a.a.

Struc: 448 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.4.1.10 - levansucrase.
• Reaction: [6)-beta-D-fructofuranosyl-(2->](n) alpha-D-glucopyranoside + sucrose = [6)-beta-D-fructofuranosyl-(2->](n+1) alpha-D-glucopyranoside + D-glucose

[6)-beta-D-fructofuranosyl-(2->](n) alpha-D-glucopyranoside + sucrose = [6)-beta-D-fructofuranosyl-(2->](n+1) alpha-D-glucopyranoside + D-glucose (Bound ligand (Het Group name = CIT) matches with 47.06% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

J Biol Chem 286:17593-17600 (2011)

PubMed id: 21454585

Polysaccharide synthesis of the levansucrase SacB from Bacillus megaterium is controlled by distinct surface motifs.

C.P.Strube, A.Homann, M.Gamer, D.Jahn, J.Seibel, D.W.Heinz.

ABSTRACT

No abstract given.