PDBsum entry 3oj2

Cytokine/signaling protein

PDB id: 3oj2

Contents

Protein chains

135 a.a.

209 a.a.

Ligands

SO4 ×5

Waters ×126

PDB id:

3oj2

Name:

Cytokine/signaling protein

Title:

Crystal structure of fgf1 complexed with the ectodomain of fgfr2b harboring the a172f pfeiffer syndrome mutation

Structure:

Heparin-binding growth factor 1. Chain: a, b. Synonym: hbgf-1, acidic fibroblast growth factor, afgf, beta- endothelial cell growth factor, ecgf-beta. Engineered: yes. Fibroblast growth factor receptor 2. Chain: c, d. Synonym: fgfr-2, keratinocyte growth factor receptor, k-sam. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: fgf1, fgfa. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: bek, fgfr2, kgfr, ksam.

Resolution:

2.20Å R-factor: 0.262 R-free: 0.294

Authors:

A.Beenken,M.Mohammadi

Key ref:

A.Beenken et al. (2012). Plasticity in interactions of fibroblast growth factor 1 (FGF1) N terminus with FGF receptors underlies promiscuity of FGF1. J Biol Chem, 287, 3067-3078. PubMed id: 22057274

Date:

20-Aug-10 Release date: 31-Aug-11

Protein chains

P05230  (FGF1_HUMAN) -  Fibroblast growth factor 1 from Homo sapiens

Seq: 155 a.a.

Struc: 135 a.a.

Protein chains

P21802  (FGFR2_HUMAN) -  Fibroblast growth factor receptor 2 from Homo sapiens

Seq: 821 a.a.

Struc: 209 a.a.*

* PDB and UniProt seqs differ at 23 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains C, D: E.C.2.7.10.1 - receptor protein-tyrosine kinase.
• Reaction: L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

J Biol Chem 287:3067-3078 (2012)

PubMed id: 22057274

Plasticity in interactions of fibroblast growth factor 1 (FGF1) N terminus with FGF receptors underlies promiscuity of FGF1.

A.Beenken, A.V.Eliseenkova, O.A.Ibrahimi, S.K.Olsen, M.Mohammadi.

ABSTRACT

No abstract given.