PDBsum entry 3ofi

Hydrolase

PDB id: 3ofi

Contents

Protein chain

954 a.a. *

Ligands

MET-GLN-ILE ×2

DIO ×3

Metals

_ZN ×2

Waters ×332

* Residue conservation analysis

PDB id:

3ofi

Name:

Hydrolase

Title:

Crystal structure of human insulin-degrading enzyme in complex with ubiquitin

Structure:

Insulin-degrading enzyme. Chain: a, b. Synonym: insulin protease, insulinase, insulysin, abeta-degrading protease. Engineered: yes. Ubiquitin. Chain: c, d. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ide. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562

Resolution:

2.35Å R-factor: 0.210 R-free: 0.240

Authors:

V.Kalas,L.A.Ralat,W.-J.Tang

Key ref:

L.A.Ralat et al. (2011). Ubiquitin is a novel substrate for human insulin-degrading enzyme. J Mol Biol, 406, 454-466. PubMed id: 21185309

Date:

15-Aug-10 Release date: 08-Sep-10

Protein chains

P14735  (IDE_HUMAN) -  Insulin-degrading enzyme from Homo sapiens

Seq: 1019 a.a.

Struc: 954 a.a.*

* PDB and UniProt seqs differ at 12 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.4.24.56 - insulysin.
• Reaction: Degradation of insulin, glucagon and other polypeptides. No action on proteins.
• Cofactor: Zn(2+)

J Mol Biol 406:454-466 (2011)

PubMed id: 21185309

Ubiquitin is a novel substrate for human insulin-degrading enzyme.

L.A.Ralat, V.Kalas, Z.Zheng, R.D.Goldman, T.R.Sosnick, W.J.Tang.

ABSTRACT

No abstract given.