PDBsum entry 3oe7

Hydrolase

PDB id: 3oe7

Contents

Protein chains

(+ 3 more) 482 a.a. *

(+ 3 more) 470 a.a. *

265 a.a. *

120 a.a. *

48 a.a. *

244 a.a. *

84 a.a. *

31 a.a. *

198 a.a. *

15 a.a. *

25 a.a. *

Ligands

ANP ×15

PO4

Metals

_MG ×15

* Residue conservation analysis

PDB id:

3oe7

Name:

Hydrolase

Title:

Structure of four mutant forms of yeast f1 atpase: gamma-i270t

Structure:

Atp synthase subunit alpha. Chain: a, b, c, j, k, l, s, t, u. Fragment: unp residues 36-545. Engineered: yes. Atp synthase subunit beta. Chain: d, e, f, m, n, o, v, w, x. Fragment: unp residues 34-511. Engineered: yes. Atp synthase subunit gamma.

Source:

Saccharomyces cerevisiae. Brewer's yeast,lager beer yeast,yeast. Organism_taxid: 4932. Gene: atp1, ybl099w, ybl0827. Expressed in: saccharomyces cerevisiae. Expression_system_taxid: 4932. Gene: atp2, yjr121w, j2041. Gene: atp3, atp3a, atp3b, ybr039w, ybr0408. Gene: atp16, ydl004w, yd8119.03, d2935.

Resolution:

3.19Å R-factor: 0.192 R-free: 0.245

Authors:

D.Arsenieva,J.Symersky,Y.Wang,V.Pagadala,D.M.Mueller

Key ref:

D.Arsenieva et al. (2010). Crystal structures of mutant forms of the yeast F1 ATPase reveal two modes of uncoupling. J Biol Chem, 285, 36561-36569. PubMed id: 20843806

Date:

12-Aug-10 Release date: 15-Sep-10

Protein chains

P07251  (ATPA_YEAST) -  ATP synthase subunit alpha, mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 545 a.a.

Struc: 482 a.a.

Protein chains

P00830  (ATPB_YEAST) -  ATP synthase subunit beta, mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 511 a.a.

Struc: 470 a.a.

Protein chain

P38077  (ATPG_YEAST) -  ATP synthase subunit gamma, mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 311 a.a.

Struc: 265 a.a.*

Protein chain

Q12165  (ATPD_YEAST) -  ATP synthase subunit delta, mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 160 a.a.

Struc: 120 a.a.

Protein chain

P21306  (ATP5E_YEAST) -  ATP synthase subunit epsilon, mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 62 a.a.

Struc: 48 a.a.

Protein chain

P38077  (ATPG_YEAST) -  ATP synthase subunit gamma, mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 311 a.a.

Struc: 244 a.a.*

Protein chain

Q12165  (ATPD_YEAST) -  ATP synthase subunit delta, mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 160 a.a.

Struc: 84 a.a.

Protein chain

P21306  (ATP5E_YEAST) -  ATP synthase subunit epsilon, mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 62 a.a.

Struc: 31 a.a.

Protein chain

P38077  (ATPG_YEAST) -  ATP synthase subunit gamma, mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 311 a.a.

Struc: 198 a.a.*

Protein chain

Q12165  (ATPD_YEAST) -  ATP synthase subunit delta, mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 160 a.a.

Struc: 15 a.a.

Protein chain

P21306  (ATP5E_YEAST) -  ATP synthase subunit epsilon, mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 62 a.a.

Struc: 25 a.a.

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chains A, B, C, G, H, I, J, K, L, P, Q, R, S, T, U, Y, Z, 1: E.C.3.6.3.14 - Transferred entry: 7.1.2.2.
• Reaction: ATP + H₂O + H⁺(In) = ADP + phosphate + H⁺(Out)

ATP + H(2)O + H(+)(In) = ADP (Bound ligand (Het Group name = ANP) matches with 81.25% similarity) + phosphate (Bound ligand (Het Group name = PO4) corresponds exactly) + H(+)(Out)

• Enzyme class 3: Chains D, E, F, M, N, O, V, W, X: E.C.7.1.2.2 - H(+)-transporting two-sector ATPase.
• Reaction: ATP + H2O + 4 H⁺(in) = ADP + phosphate + 5 H⁺(out)

ATP + H2O + 4 × H(+)(in) = ADP (Bound ligand (Het Group name = ANP) matches with 81.25% similarity) + phosphate + 5 × H(+)(out) (Bound ligand (Het Group name = PO4) corresponds exactly)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

J Biol Chem 285:36561-36569 (2010)

PubMed id: 20843806

Crystal structures of mutant forms of the yeast F1 ATPase reveal two modes of uncoupling.

D.Arsenieva, J.Symersky, Y.Wang, V.Pagadala, D.M.Mueller.

ABSTRACT

No abstract given.