PDBsum entry 3o96

Transferase

PDB id: 3o96

Contents

Protein chain

367 a.a. *

Ligands

IQO

Waters ×21

* Residue conservation analysis

PDB id:

3o96

Name:

Transferase

Title:

Crystal structure of human akt1 with an allosteric inhibitor

Structure:

Rac-alpha serine/threonine-protein kinase. Chain: a. Fragment: unp residues 2-443. Synonym: rac-pk-alpha, protein kinase b, pkb, proto-oncogenE C-akt. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: akt1, pkb, rac. Expressed in: trichoplusia ni. Expression_system_taxid: 7111. Expression_system_cell_line: hi5 insect cells

Resolution:

2.70Å R-factor: 0.249 R-free: 0.308

Authors:

W.C.Voegtli,W.-I.Wu,H.A.Lord-Ondash,F.P.Dizon,G.P.A.Vigers, B.J.Brandhuber

Key ref:

W.I.Wu et al. (2010). Crystal structure of human AKT1 with an allosteric inhibitor reveals a new mode of kinase inhibition. Plos One, 5, e12913-12913. PubMed id: 20886116

Date:

03-Aug-10 Release date: 13-Oct-10

Protein chain

P31749  (AKT1_HUMAN) -  RAC-alpha serine/threonine-protein kinase from Homo sapiens

Seq: 480 a.a.

Struc: 367 a.a.

Enzyme reactions

• Enzyme class: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Plos One 5:e12913-12913 (2010)

PubMed id: 20886116

Crystal structure of human AKT1 with an allosteric inhibitor reveals a new mode of kinase inhibition.

W.I.Wu, W.C.Voegtli, H.L.Sturgis, F.P.Dizon, G.P.Vigers, B.J.Brandhuber.

ABSTRACT

No abstract given.