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PDBsum entry 3o78
Go to PDB code: 
protein metals Protein-protein interface(s) links
Fluorescent protein PDB id
3o78

 

 

 

 

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JSmol PyMol  
Contents
Protein chains
403 a.a.
Metals
_CA ×8
Waters ×77
PDB id:
3o78
Name: Fluorescent protein
Title: The structure of ca2+ sensor (case-12)
Structure: Myosin light chain kinase, smooth muscle,green fluorescent protein,green fluorescent protein,calmodulin-1. Chain: a, b. Synonym: mlck,telokin. Engineered: yes. Mutation: yes. Other_details: chimera protein of peptide of m13 (residues 1730-1749, unp p11799), c-terminal domain of green fluorescent protein (residues 147-238, unp p42212), linker, n-terminal of green fluorescent protein
Source: Gallus gallus, aequorea victoria, homo sapiens. Chicken, jellyfish, human. Organism_taxid: 9031, 6100, 9606. Gene: mylk, gfp, calm1, calm, cam, cam1. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
2.60Å     R-factor:   0.245     R-free:   0.316
Authors: L.Leder,W.Stark,F.Freuler,M.Marsh,M.Meyerhofer,T.Stettler,L.M.Mayr, O.V.Britanova,L.A.Strukova,D.M.Chudakov
Key ref: L.Leder et al. (2010). The structure of Ca2+ sensor Case16 reveals the mechanism of reaction to low Ca2+ concentrations. Sensors (basel), 10, 8143-8160. PubMed id: 22163646
Date:
30-Jul-10     Release date:   29-Sep-10    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P0DP23  (CALM1_HUMAN) -  Calmodulin-1 from Homo sapiens
Seq:
Struc: 		149 a.a.
403 a.a.*
Protein chains
Pfam   ArchSchema ?
P11799  (MYLK_CHICK) -  Myosin light chain kinase, smooth muscle from Gallus gallus
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		1906 a.a.
403 a.a.*
Protein chains
Pfam   ArchSchema ?
P42212  (GFP_AEQVI) -  Green fluorescent protein from Aequorea victoria
Seq:
Struc: 		238 a.a.
403 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 246 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.2.7.11.18  - [myosin light-chain] kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction:
1. L-seryl-[myosin light chain] + ATP = O-phospho-L-seryl-[myosin light chain] + ADP + H+
2. L-threonyl-[myosin light chain] + ATP = O-phospho-L-threonyl-[myosin light chain] + ADP + H+
L-seryl-[myosin light chain]
 + ATP
 = O-phospho-L-seryl-[myosin light chain]
 + ADP
 + H(+)
L-threonyl-[myosin light chain]
 + ATP
 = O-phospho-L-threonyl-[myosin light chain]
 + ADP
 + H(+)
      Cofactor: Ca(2+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
Sensors (basel) 10:8143-8160 (2010)
PubMed id: 22163646  
 
 
The structure of Ca2+ sensor Case16 reveals the mechanism of reaction to low Ca2+ concentrations.
L.Leder, W.Stark, F.Freuler, M.Marsh, M.Meyerhofer, T.Stettler, L.M.Mayr, O.V.Britanova, L.A.Strukova, D.M.Chudakov, E.A.Souslova.
 
  ABSTRACT  
 
No abstract given.

 

 

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