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UniProt functional annotation for P52491 | ||
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| UniProt code: P52491. |
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| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
Accepts the ubiquitin-like protein NEDD8/RUB1 from the UBA3- ULA1 E1 complex and catalyzes its covalent attachment to other proteins. The major substrate is CDC53/Cullin. {ECO:0000269|PubMed:9545234}. |
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| Catalytic activity: | |
Reaction=[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine + [E2 NEDD8-conjugating enzyme]-L-cysteine = [E1 NEDD8-activating enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]- yl-L-cysteine.; EC=2.3.2.34; |
| Pathway: | |
Protein modification; protein neddylation. |
| Subunit: | |
Interacts with DCN1. {ECO:0000269|PubMed:21940857}. |
| Ptm: | |
The acetylation of Met-1 is cotranslational, and not regulatory. The N-acetylmethionine increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins. {ECO:0000269|PubMed:21940857}. |
| Similarity: | |
Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily. {ECO:0000255|PROSITE-ProRule:PRU00388}. |
Annotations taken from UniProtKB at the EBI.