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UniProt functional annotation for P39594 | ||
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| UniProt code: P39594. |
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| Organism: | |
Bacillus subtilis (strain 168). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus. |
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| Function: | |
Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP). Is also able to use the 2-methoxy analog MeO-HMP-PP, as substrate in vitro, but not the 2-trifluoromethyl analog CF(3)-HMP-PP. {ECO:0000269|PubMed:11513588, ECO:0000269|PubMed:9139923}. |
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| Catalytic activity: | |
Reaction=2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = CO2 + diphosphate + thiamine phosphate; Xref=Rhea:RHEA:47844, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:33019, ChEBI:CHEBI:37575, ChEBI:CHEBI:57841, ChEBI:CHEBI:62899; EC=2.5.1.3; Evidence={ECO:0000269|PubMed:9139923}; |
| Catalytic activity: | |
Reaction=2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2- methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = CO2 + diphosphate + thiamine phosphate; Xref=Rhea:RHEA:47848, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:33019, ChEBI:CHEBI:37575, ChEBI:CHEBI:57841, ChEBI:CHEBI:62890; EC=2.5.1.3; Evidence={ECO:0000269|PubMed:9139923}; |
| Catalytic activity: | |
Reaction=4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 4-methyl- 5-(2-phosphooxyethyl)-thiazole + H(+) = diphosphate + thiamine phosphate; Xref=Rhea:RHEA:22328, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:37575, ChEBI:CHEBI:57841, ChEBI:CHEBI:58296; EC=2.5.1.3; Evidence={ECO:0000269|PubMed:9139923}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:9139923}; Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000269|PubMed:9139923}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.7 uM for HMP-PP {ECO:0000269|PubMed:9139923}; KM=1.2 uM for THZ-P {ECO:0000269|PubMed:9139923}; Vmax=0.7 umol/min/mg enzyme {ECO:0000269|PubMed:9139923}; |
| Pathway: | |
Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1. |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:10350464}. |
| Induction: | |
Is weakly repressed by THZ and not at all by thiamine. {ECO:0000269|PubMed:9139923}. |
| Miscellaneous: | |
Thiamine phosphate synthase appears to proceed with a dissociative mechanism (SN1 like) in which the pyrimidine pyrophosphate dissociates to give a reactive pyrimidine intermediate which is then trapped by the thiazole moiety. |
| Similarity: | |
Belongs to the thiamine-phosphate synthase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.