PDBsum entry 3nhe

Hydrolase/protein binding

PDB id: 3nhe

Contents

Protein chains

338 a.a. *

76 a.a. *

Metals

_ZN

Waters ×508

* Residue conservation analysis

PDB id:

3nhe

Name:

Hydrolase/protein binding

Title:

High resolution structure (1.26a) of usp2a in complex with ubiquitin

Structure:

Ubiquitin carboxyl-terminal hydrolase 2. Chain: a. Fragment: unp residues 258-605. Synonym: ubiquitin thioesterase 2, ubiquitin-specific-processing protease 2, deubiquitinating enzyme 2, 41 kda ubiquitin-specific protease. Engineered: yes. Ubiquitin. Chain: b.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ubp41, usp2. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: rps27a, uba52, ubb, ubc.

Resolution:

1.26Å R-factor: 0.170 R-free: 0.189

Authors:

N.Schormann,L.J.Delucas,D.Powell Mccombs

Key ref:

N.Schormann et al. High resolution structure of usp2a in complex with ubiquitin. To be published, .

Date:

14-Jun-10 Release date: 30-Jun-10

Protein chain

O75604  (UBP2_HUMAN) -  Ubiquitin carboxyl-terminal hydrolase 2 from Homo sapiens

Seq: 605 a.a.

Struc: 338 a.a.*

Protein chain

P62979  (RS27A_HUMAN) -  Ubiquitin-ribosomal protein eS31 fusion protein from Homo sapiens

Seq: 156 a.a.

Struc: 76 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).