PDBsum entry 3nbn

Transcription/DNA

PDB id: 3nbn

Contents

Protein chains

423 a.a. *

196 a.a. *

55 a.a. *

DNA/RNA

* Residue conservation analysis

PDB id:

3nbn

Name:

Transcription/DNA

Title:

Crystal structure of a dimer of notch transcription complex trimers on hes1 DNA

Structure:

Recombining binding protein suppressor of hairless. Chain: a, d. Fragment: residues 23-448. Synonym: j kappa-recombination signal-binding protein, rbp-j kappa, rbp-jk, rbp-j, cbf-1, renal carcinoma antigen ny-ren-30. Engineered: yes. Neurogenic locus notch homolog protein 1. Chain: b, e. Fragment: residues 1872-2126.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: igkjrb, igkjrb1, rbpj, rbpjk, rbpsuh. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: notch1, tan1. Gene: kiaa0200, maml1. Synthetic: yes.

Resolution:

3.45Å R-factor: 0.256 R-free: 0.298

Authors:

K.L.Arnett,S.C.Blacklow

Key ref:

K.L.Arnett et al. (2010). Structural and mechanistic insights into cooperative assembly of dimeric Notch transcription complexes. Nat Struct Biol, 17, 1312-1317. PubMed id: 20972443

Date:

03-Jun-10 Release date: 03-Nov-10

Protein chains

Q06330  (SUH_HUMAN) -  Recombining binding protein suppressor of hairless from Homo sapiens

Seq: 500 a.a.

Struc: 423 a.a.

Protein chains

P46531  (NOTC1_HUMAN) -  Neurogenic locus notch homolog protein 1 from Homo sapiens

Seq: 2555 a.a.

Struc: 196 a.a.

Protein chains

Q92585  (MAML1_HUMAN) -  Mastermind-like protein 1 from Homo sapiens

Seq: 1016 a.a.

Struc: 55 a.a.

DNA/RNA chains

T-A-C-T-G-T-G-G-G-A-A-A-G-A-A-A-G-T-T-T-G-G-A-A-A-A-T-T-T-C-A-C-A-C-G-A-G 37 bases

A-C-T-C-G-T-G-T-G-A-A-A-C-T-T-C-C-C-A-A-A-C-T-T-T-C-T-T-T-C-C-C-A-C-A-G-T 37 bases

Nat Struct Biol 17:1312-1317 (2010)

PubMed id: 20972443

Structural and mechanistic insights into cooperative assembly of dimeric Notch transcription complexes.

K.L.Arnett, M.Hass, D.G.McArthur, M.X.Ilagan, J.C.Aster, R.Kopan, S.C.Blacklow.

ABSTRACT

No abstract given.