PDBsum entry 3n8v

Oxidoreductase

PDB id: 3n8v

Contents

Protein chains

553 a.a. *

Ligands

NAG-NDG ×2

NAG-NAG ×2

NAG-NDG-BMA-MAN-MAN

NAG-NDG-BMA-BMA

HEM ×2

BOG ×5

GOL

Waters ×224

* Residue conservation analysis

PDB id:

3n8v

Name:

Oxidoreductase

Title:

Crystal structure of unoccupied cyclooxygenase-1

Structure:

Prostaglandin g/h synthase 1. Chain: a, b. Synonym: cyclooxygenase-1, cox-1, prostaglandin-endoperoxide synthase 1, prostaglandin h2 synthase 1, pgh synthase 1, pghs-1, phs 1. Engineered: yes

Source:

Ovis aries. Sheep. Organism_taxid: 9940. Strain: sheep. Gene: cox1, ptgs1. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108.

Resolution:

3.05Å R-factor: 0.204 R-free: 0.235

Authors:

R.S.Sidhu

Key ref:

R.S.Sidhu et al. (2010). Comparison of cyclooxygenase-1 crystal structures: cross-talk between monomers comprising cyclooxygenase-1 homodimers. Biochemistry, 49, 7069-7079. PubMed id: 20669977

Date:

28-May-10 Release date: 28-Jul-10

Protein chains

P05979  (PGH1_SHEEP) -  Prostaglandin G/H synthase 1 from Ovis aries

Seq: 600 a.a.

Struc: 553 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.1.14.99.1 - prostaglandin-endoperoxide synthase.
• Reaction: (5Z,8Z,11Z,14Z)-eicosatetraenoate + AH2 + 2 O2 = prostaglandin H2 + A + H2O

(5Z,8Z,11Z,14Z)-eicosatetraenoate + AH2 + 2 × O2 = prostaglandin H2 + + H2O (Bound ligand (Het Group name = HEM) matches with 51.11% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

Biochemistry 49:7069-7079 (2010)

PubMed id: 20669977

Comparison of cyclooxygenase-1 crystal structures: cross-talk between monomers comprising cyclooxygenase-1 homodimers.

R.S.Sidhu, J.Y.Lee, C.Yuan, W.L.Smith.

ABSTRACT

No abstract given.